Reproducible enrichment of extracellular heat shock proteins from blood serum using monomeric avidin

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

卷:21

Article

Mihai, Doina M.¹  Deng, Haiteng^2,3  Kawamura, Akira¹ 

[1] CUNY Hunter Coll, Dept Chem, New York, NY 10065 USA

[2] Rockefeller Univ, Prote Resource Ctr, New York, NY 10021 USA

[3] Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China

关键词: eHsp;    Affinity resin;    Proteomics;    Serum biomarkers;    Cancer;   

DOI  :  10.1016/j.bmcl.2011.05.111

来源: Elsevier

【 摘 要 】

Extracellular heat shock proteins (eHsps) in blood circulation have been associated with various diseases, including cancer. However, the lack of methods to enrich eHsps from serum samples has hampered the characterization of eHsps. This Letter presents our serendipitous finding that the monomeric avidin resin can serve as an affinity resin to enrich eHsps from blood serum. Biochemical mechanism of this eHsp enrichment as well as implications in biomarker discovery is discussed. (C) 2011 Elsevier Ltd. All rights reserved.

【 授权许可】

Free