DNA-binding studies of the natural β-carboline eudistomin U

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

卷:26

Article

Giulietti, Jennifer M.¹  Tate, Patrick M.¹  Cai, Ang²  Cho, Bongsup²  Mulcahy, Seann P.¹ 

[1] Providence Coll, Dept Chem & Biochem, 1 Cunningham Sq, Providence, RI 02918 USA

[2] Univ Rhode Isl, Dept Biomed & Pharmaceut Sci, Coll Pharm, 7 Greenhouse Rd, Kingston, RI 02881 USA

关键词: beta-Carboline;    DNA;    Eudistomin U;    UV-Vis;    Thermal denaturation;    Circular dichroism;    Differential scanning calorimetry;   

DOI  :  10.1016/j.bmcl.2016.08.047

来源: Elsevier

【 摘 要 】

Eudistomin U is a member of the beta-carboline class of heterocyclic amine-containing molecules that are capable of binding to DNA. The structure of eudistomin U is unique since it contains an indole ring at the 1-position of the pyridine ring. While simple beta-carbolines are reported to intercalate DNA, an examination of the mode of binding of eudistomin U has been lacking. We report preliminary spectroscopic (UV-Vis, thermal denaturation, CD) and calorimetric (DSC) data on the binding of eudistomin U to DNA, which suggest that eudistomin U binds weakly according to a mechanism that is more complicated than other members of its class. (C) 2016 Elsevier Ltd. All rights reserved.

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