A high-throughput assay for O-GlcNAc transferase detects primary sequence preferences in peptide substrates

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

卷:17

Article

Leavy, Tanya M. ; Bertozzi, Carolyn R.

关键词: glycosylation;    OGT;    O-GlcNAc;    consensus sequence;    ELISA;   

DOI  :  10.1016/j.bmcl.2007.05.008

来源: Elsevier

【 摘 要 】

O-GlcNAc transferase (OGT) catalyzes the addition of N-acetylglucosamine (O-GlcNAc) onto a diverse array of intracellular proteins. Although hundreds of proteins are known to be modified by O-GlcNAc, a strict amino acid consensus sequence for OGT has not been identified. In this study, we describe the development of a high-throughput assay for OGT and use it to profile the specificity of the enzyme among a panel of peptide substrates. (C) 2007 Elsevier Ltd. All rights reserved.

【 授权许可】

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