Predicting potential miRNA target sites within gene promoters

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

卷:19

Article

Younger, Scott T.^2,3  Pertsemlidis, Alexander¹  Corey, David R.^2,3 

[1] Eugene McDermott Ctr Human Growth & Dev, Dallas, TX 75390 USA

[2] Univ Texas SW Med Ctr Dallas, Dept Pharmacol, Dallas, TX 75390 USA

[3] Univ Texas SW Med Ctr Dallas, Dept Biochem, Dallas, TX 75390 USA

关键词: RNAi;    miRNA;    MicroRNA;    Gene promoter;    Antigene;    Noncoding RNA;    Duplex RNA;    Hybridization;   

DOI  :  10.1016/j.bmcl.2009.04.032

来源: Elsevier

【 摘 要 】

Synthetic small duplex RNAs that are complementary to gene promoters can activate or inhibit target gene expression. The potency and robustness of gene modulation by these RNAs suggests that natural mechanisms may exist to facilitate recognition of sequences within gene promoters by endogenous small RNAs. Here, we describe computational methods for identifying potential miRNA target sites within gene promoters. These methods will facilitate investigations of whether miRNAs interact with sequences outside of 3'-untranslated regions and suggest new targets for the design of synthetic modulators of gene expression. (C) 2009 Elsevier Ltd. All rights reserved.

【 授权许可】

Free