Sugar Chips immobilized with synthetic sulfated disaccharides of heparin/heparan sulfate partial structure

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

卷:18

Article

Wakao, Masahiro¹  Saito, Akihiro¹  Ohishi, Koh¹  Kishimoto, Yuko²  Nishimura, Tomoaki^1,2  Sobel, Michael^3,4  Suda, Yasuo^1,2 

[1] Kagoshima Univ, Grad Sch Sci & Engn, Dept Nanostruct & Adv Mat, Kagoshima 8900065, Japan

[2] SUDx Biotec Corp, Kobe, Hyogo 6500047, Japan

[3] Univ Washington, Dept Surg, Seattle, WA 98108 USA

[4] VA Puget Sound Hlth Care Syst, Seattle, WA 98108 USA

关键词: Sugar;    carbohydrate;    chip;    heparin;    heparan sulfate;    carbohydrate-protein interaction;    surface plasmon resonance;    SPR;    SPR-imaging;   

DOI  :  10.1016/j.bmcl.2008.01.069

来源: Elsevier

【 摘 要 】

Carbohydrate chip technology has a great potential for the high-throughput evaluation of carbohydrate-protein interactions. Herein, we report syntheses of novel sulfated oligosaccharides possessing heparin and heparan sulfate partial disaccharide structures, their immobilization on gold-coated chips to prepare array-type Sugar Chips, and evaluation of binding potencies of proteins by surface plasmon resonance (SPR) imaging technology. Sulfated oligosaccharides were efficiently synthesized from glucosamine and uronic acid moieties. Synthesized sulfated oligosaccharides were then easily immobilized on gold-coated chips using previously reported methods. The effectiveness of this analytical method was confirmed in binding experiments between the chips and heparin binding proteins, fibronectin and recombinant human von Willebrand factor A1 domain ( rh-vWf-A1), where specific partial structures of heparin or heparan sulfate responsible for binding were identified. (C) 2008 Elsevier Ltd. All rights reserved.

【 授权许可】

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