Bimodal transcranial direct current stimulation reduces alcohol consumption and induces long-term neurochemical changes in rats with neuropathic pain

NEUROSCIENCE LETTERS

卷:759

Article

Santos, Daniela Silva^1,2,6,7  Medeiros, Liciane Fernandes^1,2,8  Stein, Dirson Joao^1,2,6,7  De Macedo, Isabel Cristina^1,6,7  Da Silva Rios, Diego Evandro^1,6,7  De Oliveira, Carla^1,6,7  Toledo, Roberta Stroher^1,3,6,7  Fregni, Felipe^4,5  Caumo, Wolnei²  Torres, Iraci L. S.^1,2,3,4,5 

[1] Hosp Clin Porto Alegre, Lab Pain Pharmacol & Neuromodulat Preclin Invest, Porto Alegre, RS, Brazil

[2] Univ Fed Rio Grande do Sul, Sch Med, Postgrad Program Med Sci, Porto Alegre, RS, Brazil

[3] Univ Fed Rio Grande do Sul, Inst Basic Hlth Sci, Postgrad Program Biol Sci Pharmacol & Therapeut, Porto Alegre, RS, Brazil

[4] Harvard Univ, Spaulding Rehabil Hosp, Dept Phys Med & Rehabil, Lab Neuromodulat, Boston, MA 02115 USA

[5] Harvard Univ, Massachusetts Gen Hosp, Boston, MA 02115 USA

[6] Hosp Clin Porto Alegre, Anim Expt Unit, Porto Alegre, RS, Brazil

[7] Hosp Clin Porto Alegre, Grad Res Grp, Porto Alegre, RS, Brazil

[8] Univ La Salle, Postgrad Program Hlth & Human Dev, Canoas, RS, Brazil

关键词: Chronic pain;    Alcohol;    tDCS;    Neuropeptide Y;    Cytokines;    Rats;   

DOI  :  10.1016/j.neulet.2021.136014

来源: Elsevier

【 摘 要 】

This study aimed to evaluate the effects of repeated bimodal transcranial direct current stimulation (tDCS) on alcohol consumption and immunohistological and neurochemical parameters in nerve-injured rats. Forty-eight adult male Wistar rats were distributed into six groups: control, neuropathic pain (NP) + sham-tDCS, NP + alcohol + sham-tDCS, alcohol + sham-tDCS, alcohol + tDCS, and NP + alcohol + tDCS. NP is induced by chronic sciatic nerve constriction (CCI). The rats were exposed to a 10% alcohol solution by voluntary consumption for 14 days. From the 16th day after surgery, bimodal tDCS was applied for 20 min/day for 8 days. Brain structures were collected to evaluate the number of neuropeptide Y (NPY)-positive neurons, neurites, and argyrophilic grains by immunohistochemistry, and brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), interleukin (IL)-6, and IL-10 by ELISA. Nerve-injured rats showed a progressive increase in alcohol consumption compared to the non-injured rats. In addition, there was a reduction in voluntary alcohol consumption over time induced by tDCS. Alcohol exposure, chronic pain, and tDCS treatment modulated the central NPY immunoreactivity. tDCS increased the cerebellar levels of IL-6 and IL-10, and CCI and/or tDCS reduced striatal BDNF levels. The current data suggest that tDCS could be a promising non-pharmacological adjuvant to treat patients with chronic pain who use alcohol to relieve their symptoms.

【 授权许可】

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