CaSR and calpain contribute to the ischemia reperfusion injury of spinal cord

NEUROSCIENCE LETTERS

卷:646

Article

Sun, Ji-Fu¹  Yang, Hui-Lin¹  Huang, Yong-Hui²  Chen, Qian³  Cao, Xing-Bing²  Li, Da-Peng²  Shu, Hao-Ming²  Jiang, Run-Yu² 

[1] Soochow Univ, Affiliated Hosp 1, Dept Orthoped Surg, 188 Shizi St, Suzhou 215006, Jiangsu, Peoples R China

[2] Jiangsu Univ, Affiliated Hosp, Dept Orthoped Surg, Zhenjiang, Jiangsu, Peoples R China

[3] Jiangsu Univ, Med Sch, Dept Histol & Embryol, Zhenjiang, Jiangsu, Peoples R China

关键词: Spinal cord ischemia reperfusion injury (SCIRI);    Calcium-sensing receptor (CaSR);    Calpain;    Apoptosis;   

DOI  :  10.1016/j.neulet.2017.03.009

来源: Elsevier

【 摘 要 】

Spinal cord ischemia reperfusion injury (SCIRI) can cause spinal cord dysfunction and even devastating paraplegia. Calcium-sensing receptor (CaSR) and calpain are two calcium related molecules which have been reported to be involved in the ischemia reperfusion injury of cardiomyocytes and the subsequent apoptosis. Here, we studied the expression of CaSR and calpain in spinal cord neurons and tissues, followed by the further investigation of the role of CaSR/calpain axis in the cellular apoptosis process during SCIRI. The results of in vitro and in vivo studies showed that the expression of CaSR and calpain in spinal cord neurons increased during SCIRI. Moreover, the CaSR agonist GdCl3 and antagonist NPS-2390 enhanced or decreased the expression of CaSR and calpain respectively. The expressions of CaSR and calpain were also consistent with the cellular apoptosis in spinal cord. Taken together, CaSR-calpain contributes to the SCIRI apoptosis, and CaSR antagonist might be a helpful drug for alleviating SCIRI. (C) 2017 Elsevier B.V. All rights reserved.

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