Do the accelerating actions of tianeptine and L-arginine on cortical spreading depression interact? An electrophysiological analysis in young and adult rats

NEUROSCIENCE LETTERS

卷:650

Article

Silva de Seixas Maia, Luciana Maria¹  Amancio-dos-Santos, Angela²  Pereira da Silva Germano, Paula Catirina³  Santos Marinho Falcao, Anna Carolina³  Duda-de-Oliveira, Desirre³  Araujo Guedes, Rubem Carlos³ 

[1] Univ Fed Pernambuco, Ctr Biociencias, Dept Histol & Embriol, BR-50740600 Recife, PE, Brazil

[2] Univ Fed Pernambuco, Ctr Biociencias, Dept Fisiol & Farmacol, BR-50740600 Recife, PE, Brazil

[3] Univ Fed Pernambuco, Ctr Ciencias Saude, Dept Nutr, BR-50670901 Recife, PE, Brazil

关键词: L-arginine;    Cortical spreading depression;    Nitric oxide;    Rats;    Serotonin;    Tianeptine;   

DOI  :  10.1016/j.neulet.2017.04.044

来源: Elsevier

【 摘 要 】

In the rat, we previously demonstrated that serotonin-enhancing drugs impair cortical spreading depression (CSD) and that L-arginine (arginine) treatment enhances CSD. Here, we investigated the interaction between topical application of the serotonin uptake enhancer tianeptine and systemic arginine administration on CSD. From postnatal day 7-28, female Wistar rats (n = 40) received by gavage 300 mg/Kg/day arginine (n = 20) or water (n = 20). Half of the arginine- or water-treated rats underwent CSD recording at 30-40 days of age (young), while the other half was recorded at 90-120 days (adult). Following baseline recording (four episodes of CSD), we applied tianeptine solution (10 mg/ml) to a rectangular portion of the intact dura mater for 10-min and then elicited CSD. This procedure was repeated three times. Compared to baseline values, CSD velocities and amplitudes following tianeptine application increased, and CSD duration decreased significantly (p <0.05) in both young and adult rats, regardless of treatment group. CSD acceleration caused by systemic treatment with arginine is in agreement with previous findings. Topical cortical application of tianeptine replicated the effect of systemic application, suggesting a cortically based mechanism for tianeptine's action. However, the absence of interaction between arginine and tianeptine treatments suggests that they probably act through separate mechanisms. (C) 2017 Elsevier B.V. All rights reserved.

【 授权许可】

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