Modulation of neural regulators of energy homeostasis, and of inflammation, in the pups of mice exposed to e-cigarettes

NEUROSCIENCE LETTERS

卷:684

Article

Chen, Hui^1,2,3  Li, Gerard^1,2  Chan, Yik Lung⁴  Nguyen, Tara^1,2  van Reyk, David^1,2  Saad, Sonia^1,2,5  Oliver, Brian G.^1,2,4 

[1] Univ Technol Sydney, Fac Sci, Sch Life Sci, Sydney, NSW 2007, Australia

[2] Univ Technol Sydney, Fac Sci, Ctr Hlth Technol, Sydney, NSW 2007, Australia

[3] Chengdu Univ Tradit Chinese Med, Fac Basic Med Sci, Chengdu 610072, Sichuan, Peoples R China

[4] Univ Sydney, Woolcock Inst Med Res, Resp Cellular & Mol Biol, Sydney, NSW 2037, Australia

[5] Univ Sydney, Royal North Shore Hosp, Kolling Inst Med Res, Renal Grp, Sydney, NSW 2065, Australia

关键词: Maternal smoking;    e-Cigarette;    Neuropeptide Y;    MC4R;    iNOS;   

DOI  :  10.1016/j.neulet.2018.07.001

来源: Elsevier

【 摘 要 】

Background: Maternal smoking can lead to perturbations in central metabolic regulators such as neuropeptide Y (NPY) and pro-opiomelanocortin (POMC) signalling components in offspring. With the growing interest in e-cigarettes as a tobacco replacement, this short report assessed central metabolic regulation in offspring of mouse dams exposed to e-cigarettes. We examined the impact of continuous use of e-cigarettes, and e-cigarette replacement of tobacco cigarettes during pregnancy. Supplementation of an antioxidant L-carnitine was also coused with tobacco cigarette in the mother to determine whether the impact of maternal tobacco smoking was oxidative stress driven. Methods: Balb/c mice were exposed to either nicotine-containing (E-cig18) or nicotine-free (E-cig0) e-cigarette aerosols or tobacco smoke (SE) prior to mating and until their pups were weaned. After mating, two SE subgroups were changed to E-cig18 exposure (Replacement), or supplementation L-carnitine while SE was continued. Male offspring were studied at weaning age. Results: The offspring of E-cig0 dams were the heaviest with the most body fat. Replacing SE with E-cig18 during pregnancy resulted in offspring with significantly less body fat. E-cig0 offspring had significantly increased mRNA expression of brain NPY and iNOS. Maternal SE upregulated mRNA expression of NPY, NPY Y1 receptor, POMC downstream components, and iNOS expression, which were normalised in Replacement offspring, but only partially normalised with maternal L-carnitine supplementation during gestation and lactation. Conclusions: Maternal exposure to either tobacco and nicotine-free e-cigarettes lead to disturbances in the level of central homeostatic control markers in offspring, suggesting that maternal exposure to e-cigarettes is not without risks.

【 授权许可】

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