Reduced interleukin 1A gene expression in the dorsolateral prefrontal cortex of individuals with PTSD and depression

NEUROSCIENCE LETTERS

卷:692

Article

Morrison, Filomene G.^1,2  Miller, Mark W.^1,2  Wolf, Erika J.^1,2  Logue, Mark W.^1,2,3,4  Maniates, Hannah¹  Kwasnik, David²  Cherry, Jonathan D.⁶  Svirsky, Sarah⁶  Restaino, Anthony⁶  Hildebrandt, Audrey^1,5  Aytan, Nurgul⁶  Stein, Thor D.^6,7  Alvarez, Victor E.^1,5,6  McKee, Ann C.^5,6,7  Huber, Bertrand R.^1,5,6 

[1] VA Boston Healthcare Syst, Natl Ctr PTSD, 150 South Huntington Ave, Boston, MA 02130 USA

[2] Boston Univ, Sch Med, Dept Psychiat, Boston, MA 02215 USA

[3] Boston Univ, Sch Med, Biomed Genet, Boston, MA 02215 USA

[4] Boston Univ, Dept Biostat, Sch Publ Hlth, Boston, MA 02215 USA

[5] VA Boston Healthcare Syst, Pathol & Lab Med, Boston, MA USA

[6] Boston Univ, Sch Med, Dept Neurol, Boston, MA 02215 USA

[7] Boston Univ, Sch Med, Dept Pathol, Boston, MA 02215 USA

关键词: PTSD;    Depression;    Prefrontal cortex;    Postmortem;    Interleukin;    ILIA;    Gene expression;    Inflammation;    Neuroinflammation;   

DOI  :  10.1016/j.neulet.2018.10.027

来源: Elsevier

【 摘 要 】

The inflammatory system has been implicated in the pathophysiology of a variety of psychiatric conditions. Individuals with PTSD, depression, and other fear- and anxiety-related disorders exhibit alterations in peripheral circulating inflammatory markers, suggesting dysregulation of the inflammatory system. The relationship between inflammation and PTSD has been investigated almost exclusively in the periphery, and has not been extensively explored in human postmortem brain tissue. Interleukins (ILs) represent a subtype of cytokines and are key signaling proteins in the immune and inflammatory systems. Based on prior research implicating IL signaling in PTSD and depression, we performed a preliminary investigation of IL gene expression in a region of the cortex involved in emotion regulation and PTSD, the dorsolateral prefrontal cortex (dIPFC), using tissue from the newly established VA National PTSD Brain Bank. Gene expression analyses were conducted on post-mortem tissue from the dIPFC from 50 donors: 13 controls, 12 PTSD cases, and 25 depressed cases. RNA was extracted from frozen dIPFC tissue, reverse transcribed to cDNA, and quantitative polymerase chain reaction (qPCR) was performed to assess gene expression of ILIA, ILIB, IL6, IL8, IL10, IL13, and IL15. We found a multiple-testing corrected significant decrease in ILIA expression in the dIPFC for PTSD and depression cases compared to controls (p < 0.005) with age at death, sex, race and RNA integrity number (RIN) included as covariates. To our knowledge this finding is the first demonstration of altered IL expression in brain tissue from deceased individuals with histories of PTSD and/or depression.

【 授权许可】

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