The activation of D2 and D3 receptor subtypes inhibits pathways mediating primary afferent depolarization (PAD) in the mouse spinal cord

NEUROSCIENCE LETTERS

卷:736

Article

Milla-Cruz, Jonathan J.¹  Mena-Avila, Elvia¹  Calvo, Jorge R.¹  Hochman, Shawn²  Villalon, Carlos M.³  Quevedo, Jorge N.¹ 

[1] Inst Politecn Nacl, Dept Fisiol Biofis & Neurociencias, Ctr Invest & Estudios Avanzados, Mexico City, DF, Mexico

[2] Emory Univ, Dept Physiol, Atlanta, GA 30322 USA

[3] Inst Politecn Nacl, Dept Farmacobiol, Ctr Invest & Estudios Avanzados, Mexico City, DF, Mexico

关键词: Dopamine;    Primary afferent depolarization;    Spinal cord;    Neuromodulation;    Presynaptic inhibition;   

DOI  :  10.1016/j.neulet.2020.135257

来源: Elsevier

【 摘 要 】

Somatosensory information can be modulated at the spinal cord level by primary afferent depolarization (PAD), known to produce presynaptic inhibition (PSI) by decreasing neurotransmitter release through the activation of presynaptic ionotropic receptors. Descending monoaminergic systems also modulate somatosensory processing. We investigated the role of D-1 -like and D-2 -like receptors on pathways mediating PAD in the hemisected spinal cord of neonatal mice. We recorded low-threshold evoked dorsal root potentials (DRPs) and population monosynaptic responses as extracellular field potentials (EFPs). We used a paired-pulse conditioning-test protocol to assess homosynaptic and heterosynaptic depression of evoked EFPs to discriminate between dopaminergic effects on afferent synaptic efficacy and/or on pathways mediating PAD, respectively. DA (10 mu M) depressed low-threshold evoked DRPs by 43 %, with no effect on EFPs. These depressant effects on DRPs were mimicked by the D-2-like receptor agonist quinpirole (35 %). Moreover, by using selective antagonists at D-2-like receptors (encompassing the D-2, D-3, and D-4 subtypes), we found that the D-2 and D-3 receptor subtypes participate in the quinpirole depressant inhibitory effects of pathways mediating PAD.

【 授权许可】

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