Calcium signals induced by FGF-2 in parasympathetic neurons: Role of second messenger pathways

NEUROSCIENCE LETTERS

卷:523

Article

Zamburlin, Pollyanna¹  Ruffinatti, Federico Alessandro¹  Gilardino, Alessandra^1,2  Farcito, Silvia¹  Lovisolo, Davide^1,2,3 

[1] Univ Turin, Dept Life Sci & Syst Biol, I-10123 Turin, Italy

[2] Univ Turin, NIS Interdipartimental Ctr, I-10125 Turin, Italy

[3] Univ Turin, Neurosci Inst Torino, I-10123 Turin, Italy

关键词: FGF-2;    Ciliary ganglion;    Signal transduction pathways;    Calcium signalling;   

DOI  :  10.1016/j.neulet.2012.06.035

来源: Elsevier

【 摘 要 】

Basic Fibroblast Growth Factor, or FGF-2, has been shown to promote neuronal survival and neurite outgrowth in dissociated neurons from the embryonic chick ciliary ganglion; in these effects the three main signal transduction pathways downstream the activated FGFR receptor, i.e. the MAPK, the PI3-K and the PLC gamma ones, are differentially involved. While it has been shown that FGF-2 can elicit long lasting elevations in intracellular calcium concentration, [Ca2+](i), the role of the three pathways in this process has not been elucidated. Here we show, by means of pharmacological inhibitors, that all three are involved, at a different extent, in the generation of the [Ca2+](i); increase induced by FGF-2; in particular, inhibition of the PLC gamma pathway, in addition to reducing the number of responsive cells, induces, in a significant population of cells, basal calcium oscillations in the absence of the growth factor and interferes with calcium signals elicited by depolarization. We propose that this complex behaviour can be due to a perturbation in PIP2 levels at the plasmamembrane. (C) 2012 Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

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