Brain injury results in lower levels of melatonin receptors subtypes MT1 and MT2

NEUROSCIENCE LETTERS

卷:650

Article

Osier, Nicole D.^1,2  Pham, Lan¹  Pugh, Bunny J.²  Puccio, Ava^1,3  Ren, Dianxu¹  Conley, Yvette P.^1,4  Alexander, Sheila^1,5  Dixon, C. Edward^2,3,6 

[1] Univ Pittsburgh, Sch Nursing, Victoria Bldg,3500 Victoria St, Pittsburgh, PA 15213 USA

[2] Childrens Hosp Pittsburgh UPMC, Safar Ctr Resuscitat Res, John G Rangos Res, Ctr 6th Floor,4401 Penn Ave, Pittsburgh, PA 15224 USA

[3] Univ Pittsburgh, Dept Neurol Surg, Brain Trauma Res Ctr, UPMC Presbyterian, Suite B-400,200 Lothrop St, Pittsburgh, PA 15213 USA

[4] Univ Pittsburgh, Dept Human Genet, Crabtree Hall,130 De Soto St, Pittsburgh, PA 15261 USA

[5] Univ Pittsburgh, Sch Med, M240 Scaife Hall,3550 Terrace St, Pittsburgh, PA 15261 USA

[6] VA Pittsburgh Healthcare Syst, 4100 Allequippa St, Pittsburgh, PA 15261 USA

关键词: Traumatic brain injury (TBI);    Brain trauma;    Controlled cortical impact (CCI);    Rat;    Melatonin;    Receptors;   

DOI  :  10.1016/j.neulet.2017.03.053

来源: Elsevier

【 摘 要 】

Background: Traumatic brain injury (TBI) is a devastating and costly acquired condition that affects individuals of all ages, races, and geographies via a number of mechanisms. The effects of TBI on melatonin receptors remain unknown. Purpose: The purpose of this study is to explore whether endogenous changes in two melatonin receptor subtypes (MT1 and MT2) occur after experimental TBI. Sample: A total of 25 adult male Sprague Dawley rats were used with 6 or 7 rats per group. Methods: Rats were randomly assigned to receive either TBI modeled using controlled cortical impact or sham surgery and to be sacrificed at either 6- or 24-h post-operatively. Brains were harvested, dissected, and flash frozen until whole cell lysates were prepared, and the supernatant fluid aliquoted and used for western blotting. Primary antibodies were used to probe for melatonin receptors (MT1 and MT2), and beta actin, used for a loading control. Image) and Image Lab software were used to quantify the data which was analyzed using t-tests to compare means. Results: Melatonin receptor levels were reduced in a brain region- and time point- dependent manner. Both MT1 and MT2 were reduced in the frontal cortex at 24 h and in the hippocampus at both 6 h and 24 h. Discussion: MT1 and MT2 are less abundant after injury, which may alter response to MEL therapy. Studies characterizing MT1 and MT2 after TBI are needed, including exploration of the time course and regional patterns, replication in diverse samples, and use of additional variables, especially sleep-related outcomes. Conclusion: TBI in rats resulted in lower levels of MT1 and MT2; replication of these findings is necessary as is evaluation of the consequences of lower receptor levels. (C) 2017 Elsevier B.V. All rights reserved.

【 授权许可】

Free