Cortical contusion injury disrupts olfactory bulb neurogenesis in adult mice

BMC Neuroscience

Martin L Doughty¹  Kevin J Yi¹  Qiong Zhou¹  Kryslaine L Radomski¹ 

[1] Center for Neuroscience and Regenerative Medicine (CNRM), Department of Anatomy, Physiology and Genetics, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA

关键词: Olfactory bulb;    Neural stem cell (NSC);    Subventricular zone (SVZ);    Controlled cortical impact (CCI);    Traumatic brain injury (TBI);   

Others  :  1131320
DOI  :  10.1186/1471-2202-14-142

 received in 2013-07-09, accepted in 2013-11-08,  发布年份 2013

【 摘 要 】

Background

Experimental brain trauma activates quiescent neural stem cells (NSCs) to increase neuronal progenitor cell proliferation in the adult rodent brain. Previous studies have shown focal brain contusion in the form of a unilateral controlled cortical impact (CCI) stimulates NSCs to bilaterally increase neurogenesis in the adult hippocampus.

Results

In this study we clarified the bi-lateral effects of a unilateral CCI on proliferation in the subventricular zone (SVZ) NSC niche and on neurogenesis in the olfactory bulb of adult mice. By varying the depth of impact from 1 mm to 2 mm depth, we show CCI to the left somatosensory cortex resulted in graded changes in mouse behavior and cellular pathology in the forebrain. As expected, contusion to the sensorimotor cortex resulted in motor coordination deficits in adult mice. During the first 3 days after injury, CCI increased proliferation in the impacted cortex, deeper striatum and SVZ of the forebrain ipsilateral to the CCI. In each of these regions proliferation was increased with increasing injury severity. At 30 days post-procedure, CCI resulted in a significant reduction in neurogenesis in the olfactory bulb ipsilateral to the CCI. Olfactory avoidance testing indicated disruptions in olfactory bulb neurogenesis were associated with impaired olfactory discrimination in mice post-injury.

Conclusion

The data demonstrate a focal cortical contusion injury to the left somatosensory cortex disrupts SVZ-olfactory bulb neurogenesis and impairs olfactory discrimination and motor coordination in adult mice.

【 授权许可】

   
2013 Radomski et al.; licensee BioMed Central Ltd.

【 图 表 】

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