Expression of transient receptor potential (TRP) channel mRNAs in the mouse olfactory bulb

NEUROSCIENCE LETTERS

卷:524

Article

Dong, Hong-Wei¹  Davis, James C.¹  Ding, ShengYuan²  Nai, Qiang¹  Zhou, Fu-Ming²  Ennis, Matthew¹ 

[1] Univ Tennessee, Hlth Sci Ctr, Dept Anat & Neurobiol, Memphis, TN 38163 USA

[2] Univ Tennessee, Hlth Sci Ctr, Dept Pharmacol, Memphis, TN 38163 USA

关键词: Transient receptor potential (TRP) channel;    Olfactory bulb;    External tufted cell;    Mitral cell;    Granule cell;    RT-PCR;   

DOI  :  10.1016/j.neulet.2012.07.013

来源: Elsevier

【 摘 要 】

Transient receptor potential (TRP) channels are a large family of cation channels. The 28 TRP channel subtypes in rodent are divided into 6 subfamilies: TRPC1-7, TRPV1-6, TRPM1-8, TRPP2/3/5, TRPML1-3 and TRPA1. TRP channels are involved in peripheral olfactory transduction. Several TRPC channels are expressed in unidentified neurons in the main olfactory bulb (OB), but the expression of most TRP channels in the OB has not been investigated. The present study employed RT-PCR as an initial survey of the expression of TRP channel mRNAs in the mouse OB and in 3 cell types: external tufted, mitral and granule cells. All TRP channel mRNAs except TRPV5 were detected in OB tissue. Single cell RT-PCR revealed that external tufted, mitral and granule cell populations expressed in aggregate 14 TRP channel mRNAs encompassing members of all 6 subfamilies. These different OB neuron populations expressed 7-12 channel mRNAs. Common channel expression was more similar among external tufted and mitral cells than among these cells and granule cells. These results indicate that a large number of TRP channel subtypes are expressed in OB neurons, providing the molecular bases for these channels to regulate OB neuron activity and central olfactory processing. Published by Elsevier Ireland Ltd.

【 授权许可】

Free