NFκB1 and NFκB2 gene expression in the prefrontal cortex and hippocampus of early life stressed mice exposed to cocaine-induced conditioned place preference during adolescence

NEUROSCIENCE LETTERS

卷:658

Article

Orso, Rodrigo^1,3  Creutzberg, Kerstin Camile¹  Centeno-Silva, Anderson¹  Carapecos, Matheus Silva¹  Levandowski, Mateus Luz^1,2  Wearick-Silva, Luis Eduardo^1,3  Viola, Thiago Wendt^1,3  Grassi-Oliveira, Rodrigo^1,2,3 

[1] Pontifical Catholic Univ Rio Grande do Sul PUCRS, Brain Inst Rio Grande do Sul InsCer, DCNL, Porto Alegre, RS, Brazil

[2] Pontifical Catholic Univ Rio Grande do Sul PUCRS, Grad Program Psychol, Porto Alegre, RS, Brazil

[3] Pontifical Catholic Univ Rio Grande do Sul PUCRS, Grad Program Pediat & Child Hlth, Porto Alegre, RS, Brazil

关键词: Cocaine;    Conditioned place preference;    Early life stress;    Maternal separation;    NFIcB mRNA;   

DOI  :  10.1016/j.neulet.2017.08.032

来源: Elsevier

【 摘 要 】

Neuro-immune pathways contribute to the onset and maintenance of cocaine-seeking behaviors, particularly through activation of NF kappa B signaling in the brain. However, the molecular mechanisms of this relationship are still not completely understood, especially considering the effects of early life stress, a major risk factor to cocaine addiction. The goal of this study was to investigate NF kappa B1 and NF kappa B2 gene expression in the prefrontal cortex (PFC) and hippocampus of mice exposed to early life stress and cocaine-induced conditioned place preference (CPP) within adolescence. Male BALB/c mice were randomly assigned to one of four groups: animal facility reared (AFR) with or without CPP training; maternal separation (MS) with our without CPP training. The MS animals were subjected to daily 3-h maternal separation from postnatal day (PND) 2 to 15. CPP was performed following three sequential phases: habituation (PND 34), conditioning (PND 35 to PND 44) and post conditioning test (PND 45). Gene expression was determined by qPCR. NF kappa B1 mRNA levels were decreased in the PFC of animals exposed to CPP compared to drug-nave animals, while no difference was detected regarding rearing conditions. NF kappa B2 expression was upregulated in the PFC of animals exposed to CPP when compared to drug-naive animals, particularly in animals exposed to MS with higher CPP scores. No significant effects were detected in the hippocampus. Cortical NF kappa B2 up-regulation may be involved with the enhanced motivational salience for cocaine-paired cues observed in animals exposed to MS during adolescence.

【 授权许可】

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