Neonatal morphine exposure and maternal deprivation alter nociceptive response and central biomarkers' levels throughout the life of rats

NEUROSCIENCE LETTERS

卷:738

Article

Oliveira, Carla^1,2  Toledo, Roberta Stroher^1,3  Scarabelot, Vanessa Leal¹  Vercelino, Rafael¹  da Silva, Lisiane Santos^1,4  Regner, Gabriela Gregory¹  de Souza, Andressa¹  Silveira, Natalia Paula¹  Caumo, Wolnei⁴  Torres, Iraci L. S.^1,3,4 

[1] Hosp Clin Porto Alegre HCPA, Ctr Pesquisa Expt, Lab Farmacol Dor & Neuromodulacao Invest Preclin, Porto Alegre, RS, Brazil

[2] Fac Sao Francisco Assis UNIFIN, Porto Alegre, RS, Brazil

[3] Univ Fed Rio Grande do Sul UFRGS, Inst Ciencias Basicas Saude, Programa Posgrad Ciencias Biol Farmacol & Terapeu, Porto Alegre, RS, Brazil

[4] Univ Fed Rio Grande do Sul UFRGS, Programa Posgrad Ciencias Med, Porto Alegre, RS, Brazil

关键词: opioid;    maternal-separation;    neonate rats;    nociception;   

DOI  :  10.1016/j.neulet.2020.135350

来源: Elsevier

【 摘 要 】

In the present study, we investigated the effect of repeated neonatal morphine exposure and/or maternal deprivation(MD) on the nociceptive response and central biomarkers' BDNF, IL-1 beta, and IL-4 levels at postnatal days 16(PND16), 30(PND30), and 60(PND60). At birth, the litters were standardized to contain 8 pups/dam (n = 58). From PND1 to PND10, the pups of the deprived groups were separated daily from their mothers for 3 h and divided into 5 groups: control(C), saline(S), morphine(M), deprived-saline(DS), and deprived-morphine (DM). The pups received subcutaneous injections of saline/morphine (5 mu g) in the mid-scapular area between PND8 and PND14. Nociceptive responses were assessed by hot plate(HP) and tail-flick(TFL) tests and biomarker levels by ELISA. Thermal hyperalgesia(HP) was found in all assessments for the M, DS, and DM groups, and a decrease in nociceptive threshold(TFL) was found in the DS group at PND16; M and DM groups at PND30; and M, DS, and DM groups at PND60. There were interactions between treatment/deprivation/timepoint in all central biomarkers' levels. The current study indicates that neonatal exposure to morphine and MD, which occurs in the pediatric ICU, can alter the nociceptive and neuroinflammatory responses.

【 授权许可】

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