Effect of N-acetylcysteine on the spinal-cord glutathione system and nitric-oxide metabolites in rats with neuropathic pain

NEUROSCIENCE LETTERS

卷:569

Article

Horst, Andrea¹  Kolberg, Carolina¹  Moraes, Maira S.¹  Riffel, Ana Paula K.²  Finamor, Isabela A.²  Bello-Klein, Adriane¹  Pavanato, Maria Amalia²  Partata, Wania A.¹ 

[1] Univ Fed Rio Grande do Sul, Inst Ciencias Basicas Saude, Dept Fisiol, Porto Alegre, RS, Brazil

[2] Univ Fed Santa Maria, Dept Farmacol & Fisiol, BR-97119900 Santa Maria, RS, Brazil

关键词: Glutathione peroxidase;    Glutathione-S-transferase;    Oxidized and reduced glutathione;    Hydrogen peroxide;    Chronic constriction;    Sciatic nerve;   

DOI  :  10.1016/j.neulet.2014.03.063

来源: Elsevier

【 摘 要 】

Since N-acetylcysteine (NAC) is a donor of cysteine, we studied the relationship between NAC and concentration of oxidized and reduced glutathione (GSH/GSSG ratio), and glutathione peroxidase (GPx) and glutathione-S-transferase (GST) activities in the lumbosacral spinal cord of rats with chronic constriction injury (CCI) of the sciatic nerve that received NAC (150 mg/kg/day, i.p.) or 0.9% saline solution for 3 or 10 days. Hydrogen peroxide (H2O2) and nitric-oxide (NO) metabolites were also measured. Von Frey hair and hot-plate tests showed hyperalgesia at day 1 in CCI rats. Hyperalgesia persisted at all other times in saline-treated CCI rats, but returned to pre-injury values in NAC-treated CCI rats after 3 postoperative days. GST activity and the GSH/GSSG ratio increased in saline-treated CCI rats, while the NAC treatment increased GST and GPx activities at day 10, with no significant change in the GSH/GSSG ratio. NAC treatment did not affect H2O2 levels, but it reduced NO metabolites in CCI rats 3 days after the surgery. Thus, the anti-hyperalgesic effect of NAC appears not to involve its action as a cysteine precursor for GSH synthesis, but involves a decrease in NO. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

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