Effects of voluntary alcohol drinking on corticotropin-releasing factor and preprodynorphin mRNA levels in the central amygdala of Sardinian alcohol-preferring rats

NEUROSCIENCE LETTERS

卷:554

Article

Zhou, Yan¹  Colombo, Giancarlo²  Gessa, Gian Luigi²  Kreek, Mary Jeanne¹ 

[1] Rockefeller Univ, Lab Biol Addict Dis, New York, NY 10065 USA

[2] Natl Res Council Italy, Inst Neurosci, Sect Cagliari, I-09042 Monserrato, CA, Italy

关键词: Corticotropin-releasing factor;    Preprodynorphin;    Central amygdala;    Alcohol drinking;    Sardinian alcohol-preferring (sP) and -nonpreferring (sNP) rats;    Gene expression;   

DOI  :  10.1016/j.neulet.2013.08.071

来源: Elsevier

【 摘 要 】

The stress-response corticotropin-releasing factor (CRF) and dynorphin systems are critically involved in alcohol drinking and anxiety-related behaviors. Selectively bred Sardinian alcohol-preferring (sP) rats display high inherent anxiety-related behaviors, in comparison with their alcohol-nonpreferring counterpart (sNP rats). The present study was undertaken to investigate: (1) if there were genetically determined differences in basal gene expression levels of CRF, CRF-R1, preprodynorphin (ppDyn) and kappa opioid receptor (KOP-r) between sP and sNP rats; specifically, mRNA levels of the above genes were measured in the central amygdala (CeA), hypothalamus and other stress responsive and mesolimbic regions of alcohol-naive sP and sNP rats; and (2) if the above mRNA levels were altered by voluntary alcohol drinking in sP rats exposed to the standard, homecage 2-bottle alcohol vs. water choice regimen 24 h/day for 17 days. Higher basal CRF mRNA levels were found only in CeA of alcohol-naive sP rats, compared with sNP rats; these levels were decreased after alcohol consumption. In contrast, ppDyn mRNA levels in CeA of sP rats were increased by alcohol consumption, but with no basal difference from sNP rats. Although higher basal ppDyn mRNA levels were found in hypothalamus of sP rats, compared with sNP rats, there was no alteration after alcohol drinking in sP rats. No difference for the above mRNA levels was observed in other regions, including nucleus accumbens shell or core, caudate-putamen, ventral tegmental area and medial/basolateral amygdala, between the two rat lines before or after alcohol consumption. Our results demonstrate the existence of genetically determined high basal CRF mRNA levels in CeA of sP rats. Alcohol consumption decreased CeA CRF mRNA levels with parallel increases in CeA ppDyn mRNA levels. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

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