A role for DJ-1 against oxidative stress in the mammalian retina

NEUROSCIENCE LETTERS

卷:708

Article

Martin-Nieto, Jose^1,2  Luz Uribe, Mary^1,4  Esteve-Rudd, Julian^1,5  Trinidad Herrero, Maria³  Canapello, Laura^1,6 

[1] Univ Alicante, Fac Ciencias, Dept Fisiol Genet & Microbiol, Campus Univ San Vicente, E-03080 Alicante, Spain

[2] Univ Alicante, Inst Multidisciplinar Estudio Medio Ramon Margale, E-03080 Alicante, Spain

[3] Univ Murcia, IMIB, Neurociencia Clin & Expt NiCE, Fac Med,Inst Invest Envejecimiento, E-30071 Murcia, Spain

[4] Weizmann Inst Sci, Fac Biol, Dept Biol Regulat, IL-76100 Rehovot, Israel

[5] Bayer AG, D-42096 Wuppertal, Germany

[6] NEI, Neurobiol Neurodegenerat & Repair Lab, NIH, Bethesda, MD 20892 USA

关键词: Parkinson disease;    DJ-1;    Retina;    Gene expression;    Oxidative stress;    Rotenone;   

DOI  :  10.1016/j.neulet.2019.134361

来源: Elsevier

【 摘 要 】

We have previously reported the expression of Parkinson disease-associated genes encoding alpha-synuclein, parkin and UCH-L1 in the retina across mammals. DJ-1, or parkinsonism-associated deglycase, is a redox-sensitive protein with putative roles in cellular protection against oxidative stress, among a variety of functions, acting through distinct pathways and mechanisms in a wide variety of tissues. Its function in counteracting oxidative stress in the retina, as it occurs in Parkinson and other human neurodegenerative diseases, is, however, poorly understood. In the present study, we address the expression of DJ-1 in the mammalian retina and its putative neuroprotective role in this tissue in a well-known model of parkinsonism, the rotenone-treated rat. As a result, we demonstrate that the DJ1 gene is expressed at both mRNA and protein levels in the neural retina and retinal pigment epithelium (RPE) of all mammalian species studied. We also present evidence that DJ-1 functions in the retina as a sensor of cellular redox homeostasis, which reacts to oxidative stress by increasing its intracellular levels and additionally becoming oxidized. Levels of alpha-synuclein also became upregulated, although parkin and UCH-L1 expression remained unchanged. It is inferred that DJ-1 likely exerts in the retina a potential neuroprotective role against oxidative stress, including alpha-synuclein oxidation and aggregation, which should be operative under both physiological and pathological conditions.

【 授权许可】

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