A Cellular Potts energy-based approach to analyse the influence of the surface topography on single cell motility

JOURNAL OF THEORETICAL BIOLOGY

卷:509

Article

Thenard, Thomas^1,2  Catapano, Anita²  Mesnard, Michel²  Allena, Rachele¹ 

[1] HESAM Univ, Arts & Metiers Inst Technol, IBHGC, Sorbonne Paris Cite,Univ Paris 13, F-75013 Paris, France

[2] HESAM Univ, Univ Bordeaux, Arts & Metiers Inst Technol, INRA,I2M UMR 5295,Bordeaux INP,CNRS, F-33405 Talence, France

关键词: Cell migration;    Cellular Potts model;    Surface roughness;    Contact surface;   

DOI  :  10.1016/j.jtbi.2020.110487

来源: Elsevier

【 摘 要 】

The surface shape is an important aspect to take into account to ensure the success of an implant. At the cellular scale level, the cell behaviour, especially its migration, is affected by the specificities of the surface of the substrate, such as the stiffness of the surface and its roughness topography. The latter has been shown to have a great impact on various cell mechanisms, such as the cell adhesion, migration, or proliferation. In fact, the mere presence of micro roughness leads to an improvement of those mechanisms, with a better integration of the implants. However, the phenomena behind those improvements are still not clear. In this paper, we propose a three-dimensional (3D) model of a single cell migration using a Cellular Potts (CP) model to study the influence of the surface topography on cell motility. To do so, various configurations were tested, such as: (i) a substrate with a random roughness, (ii) a substrate with a rectangular groove pattern (parallel and perpendicular to the direction of motion), (ii) a substrate with a sinusoidal groove pattern. To evaluate the influence of the surface topography on cell motility, for each configuration, the cell speed and shape as well as the contact surface between the cell and the substrate have been quantified. Our numerical results demonstrate that, in agreement with the experimental observations of the literature, the substrate topography has an influence on the cell efficiency (i.e. cell speed), orientation and shape. Besides, we also show that the increase of the contact surface alone in presence of roughness is not enough to explain the improvement of cell migration on the various rough surfaces. Finally, we highlight the importance of the roughness dimension on cell motility. This could be a critical aspect to consider for further analyses and applications, such as surface treatments for medical applications. (C) 2020 Published by Elsevier Ltd.

【 授权许可】

Free