Acetylcholinesterase is associated with a decrease in cell proliferation of hepatocellular carcinoma cells

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

卷:1852

Article

Perez-Aguilar, Benjamin^1,2  Vidal, Cecilio J.³  Palomec, Guillermina⁴  Garcia-Dolores, Fernando⁵  Concepcion Gutierrez-Ruiz, Maria²  Bucio, Leticia²  Luis Gomez-Olivares, Jose⁴  Enrique Gomez-Quiroz, Luis² 

[1] Univ Autonoma Metropolitana Iztapalapa, Biol Expt, Mexico City 09340, DF, Mexico

[2] Univ Autonoma Metropolitana Iztapalapa, Dept Ciencias Salud, Lab Fis Celular, Mexico City 09340, DF, Mexico

[3] Univ Murcia, Dept Bioquim & Biol Mol A, IMIB Arrixaca, Murcia, Spain

[4] Univ Autonoma Metropolitana Iztapalapa, Dept Ciencias Salud, Lab Biomembranas, Mexico City 09340, DF, Mexico

[5] Inst Ciencias Forenses, Lab Patol, Mexico City, DF, Mexico

关键词: Acetylcholinesterase;    Cell cycle;    HCC;    HepG2 cell;    Huh-7 cell;    Liver;   

DOI  :  10.1016/j.bbadis.2015.04.003

来源: Elsevier

【 摘 要 】

Acetylcholinesterase (AChE), the enzyme that rapidly splits acetylcholine into acetate and choline, presents non-cholinergic functions through which may participate in the control of cell proliferation and apoptosis. These two features are relevant in cancer, particularly in hepatocellular carcinoma (HCC), a very aggressive liver tumor with high incidence and poor prognosis in advanced stages. Here we explored the relation between acetylcholinesterase and HCC growth by testing the influence of AChE on proliferation of Huh-7 and HepG2 cell lines, addressed in monolayer cultures, spheroid formation and human liver tumor samples. Results showed a clear relation in AChE expression and cell cycle progression, an effect which depended on cell confluence. Inhibition of AChE activity led to an increase in cell proliferation, which was associated with downregulation of p27 and cyclins. The fact that Huh-7 and HepG2 cell lines provided similar results lent weight to the relationship of AChE expression with cell cycle progression in hepatoma cell lines at least. Human liver tumor samples exhibited a decrease in AChE activity as compared with normal tissue. The evidence presented herein provides additional support for the proposed tumor suppressor role of AChE, which makes it a potential therapeutic target in therapies against hepatocellular carcinoma. (C) 2015 Elsevier B.V. All rights reserved.

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