Cloning and expression of the dihydroorotate dehydrogenase from Toxoplasma gondii

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

卷:1637

Article

Pagan, MLS ; Zimmermann, BH

关键词: dihydroorotate dehydrogenase;    Toxoplasma gondii;    pyrimidine biosynthesis;   

DOI  :  10.1016/S0925-4439(02)00226-0

来源: Elsevier

【 摘 要 】

A full-length dihydroorotate dehydrogenase (DHODase) sequence was cloned from a Toxoplasma gondii tachyzoite cDNA library. The sequence was most similar to family 2 DHODases, and had a calculated molecular mass of 65.1 kDa. The full-length and two N-terminally truncated T. gondii DHODase sequences were expressed as recombinant proteins. One of the truncated sequences complemented a DHODase-deficient bacterial host. (C) 2002 Elsevier Science B.V. All rights reserved.

【 授权许可】

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