Niemann-Pick Type C1 deficiency in microglia does not cause neuron death in vitro

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

卷:1812

Article

Peake, Kyle B.^1,2  Campenot, Robert B.³  Vance, Dennis E.^1,4  Vance, Jean E.^1,2 

[1] Univ Alberta, Grp Mol & Cell Biol Lipids, Edmonton, AB T6G 2S2, Canada

[2] Univ Alberta, Dept Med, Edmonton, AB T6G 2S2, Canada

[3] Univ Alberta, Dept Cell Biol, Edmonton, AB T6G 2S2, Canada

[4] Univ Alberta, Dept Biochem, Edmonton, AB T6G 2S2, Canada

关键词: Niemann-Pick Type C;    Microglia;    Tumor necrosis factor;    Interleukin-10;    Neurodegeneration;   

DOI  :  10.1016/j.bbadis.2011.06.003

来源: Elsevier

【 摘 要 】

Niemann-Pick Type C (NPC) disease is an autosomal recessive disorder that results in accumulation of cholesterol and other lipids in late endosomes/lysosomes and leads to progressive neurodegeneration and premature death. The mechanism by which lipid accumulation causes neurodegeneration remains unclear. Inappropriate activation of microglia, the resident immune cells of the central nervous system, has been implicated in several neurodegenerative disorders including NK disease. Immunohistochemical analysis demonstrates that NPC1 deficiency in mouse brains alters microglial morphology and increases the number of microglia. In primary cultures of microglia from Npc1(-/-) mice cholesterol is sequestered intracellularly, as occurs in other NPC-deficient cells. Activated microglia secrete potentially neurotoxic molecules such as tumor necrosis factor-alpha (TNF alpha). However, NPC1 deficiency in isolated microglia did not increase TNF alpha mRNA or INF alpha secretion in vitro. In addition, qPCR analysis shows that expression of pro-inflammatory and oxidative stress genes is the same in Npc1(+/+) and Nod microglia, whereas the mRNA encoding the anti-inflammatory cytokine, interleukin-10 in Npc1(-/-) microglia is similar to 60% lower than in Npc1(+/+) microglia. The survival of cultured neurons was not impaired by NPC1 deficiency, nor was death of Npc1(-/-) and Npc1(+/+) neurons in microglia-neuron co-cultures increased by NPC1 deficiency in microglia. However, a high concentration of Npc1(-/-) microglia appeared to promote neuron survival. Thus, although microglia exhibit an active morphology in NPC1-deficient brains, lack of NPC1 in microglia does not promote neuron death in vitro in microglia-neuron co-cultures, supporting the view that microglial NPC1 deficiency is not the primary cause of neuron death in NK disease. (C) 2011 Elsevier B.V. All rights reserved.

【 授权许可】

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