The combination of Bifidobacterium breve with non-digestible oligosaccharides suppresses airway inflammation in a murine model for chronic asthma

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

卷:1842

Article

Sagar, Seil^1,2  Vos, Arjan P.^1,2  Morgan, Mary E.¹  Garssen, Johan^1,2  Georgiou, Niki A.²  Boon, Louis³  Kraneveld, Aletta D.¹  Folkerts, Gert¹ 

[1] Univ Utrecht, Div Pharmacol, Utrecht Inst Pharmaceut Sci, Fac Sci, NL-3508 TB Utrecht, Netherlands

[2] Danone Res, Ctr Specialised Nutr, Wageningen, Netherlands

[3] Bioceros BV, Utrecht, Netherlands

关键词: Allergic asthma;    Airway inflammation;    Bifidobacterium breve;    Murine asthma model;    Regulatory T cell;    Transcription factor;   

DOI  :  10.1016/j.bbadis.2014.01.005

来源: Elsevier

【 摘 要 】

Over the last decade, there has been a growing interest in the use of interventions that target the intestinal microbiota as a treatment approach for asthma. This study is aimed at exploring the therapeutic effects of long-term treatment with a combination of Bifidobacterium breve with non-digestible oligosaccharides on airway inflammation and remodeling. A murine ovalbumin-induced chronic asthma model was used. Pulmonary airway inflammation; mRNA expression of pattern recognition receptors, Th-specific cytokines and transcription factors in lung tissue; expression of Foxp3 in blood Th cells; in vitro T cell activation; mast cell degranulation; and airway remodeling were examined. The combination of B. breve with non-digestible oligosaccharides suppressed pulmonary airway inflammation; reduced T cell activation and mast cell degranulation; modulated expression of pattern recognition receptors, cytokines and transcription factors; and reduced airway remodeling. The treatment induced regulatory T cell responses, as shown by increased Il10 and Foxp3 transcription in lung tissue, and augmented Foxp3 protein expression in blood CD4+CD25+Foxp3 + T cells. This specific combination of beneficial bacteria with non-digestible oligosaccharides has strong anti-inflammatory properties, possibly via the induction of a regulatory T cell response, resulting in reduced airway remodeling and, therefore, may be beneficial in the treatment of chronic inflammation in allergic asthma. (C) 2014 Elsevier B.V. All rights reserved.

【 授权许可】

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