Oral immunotherapy with the ingestion of house dust mite extract in a murine model of allergic asthma

Allergy, Asthma & Clinical Immunology

Hsiao-Ling Chen¹  Chuan-Mu Chen²  Tung-Chou Tsai²  Yen-Ching Lee²  Hui-Chen Chen³  Hueng-Chuen Fan⁴  Yao-Tung Wang⁵  Hsu-Chung Liu⁶ 

[1] Department of Bioresources, Da-Yeh University;Department of Life Sciences, College of Life Sciences, National Chung Hsing University;Department of Microbiology and Immunology, School of Medicine, China Medical University;Department of Pediatrics, Tungs’ Taichung Metroharbor Hospital;Division of Pulmonary Medicine, Department of Internal Medicine, Chung Shan Medical University Hospital;School of Medicine, Chung Shan Medical University;

关键词: Allergen-specific immunotherapy;    House dust mite;    Allergic asthma;    Oral immunotherapy;    Airway inflammation;   

DOI  :  10.1186/s13223-018-0269-2

来源: DOAJ

【 摘 要 】

Abstract Background Allergen-specific immunotherapy (ASIT) has the potential to modify allergic diseases, and it is also considered a potential therapy for allergic asthma. House dust mite (HDM) allergens, a common source of airborne allergen in human diseases, have been developed as an immunotherapy for patients with allergic asthma via the subcutaneous and sublingual routes. Oral immunotherapy with repeated allergen ingestion is emerging as another potential modality of ASIT. The aim of this study was to evaluate the therapeutic efficacy of the oral ingestion of HDM extracts in a murine model of allergic asthma. Methods BABL/c mice were sensitized twice by intraperitoneal injection of HDM extracts and Al(OH)3 on day 1 and day 8. Then, the mice received challenge to induce airway inflammation by intratracheal instillation of HDM extracts on days 29–31. The treatment group received immunotherapy with oral HDM extracts ingestion before the challenge. All the mice were sacrificed on day 32 for bronchoalveolar inflammatory cytokines, mediastinal lymph node T cells, lung histology, and serum HDM-specific immunoglobulins analyses. Results Upon HDM sensitization and following challenge, a robust Th2 cell response and eosinophilic airway inflammation were observed in mice of the positive control group. The mice treated with HDM extracts ingestion had decreased eosinophilic airway inflammation, suppressed HDM-specific Th2 cell responses in the mediastinal lymph nodes, and attenuated serum HDM-specific IgE levels. Conclusions Oral immunotherapy with HDM extracts ingestion was demonstrated to have a partial therapeutic effect in the murine model of allergic asthma. This study may serve as the basis for the further development of oral immunotherapy with HDM extracts in allergic asthma.

【 授权许可】

Unknown