MicroRNA-125b inhibits lens epithelial cell apoptosis by targeting p53 in age-related cataract

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

卷:1842

Article

Qin, Yu¹  Zhao, Jiangyue¹  Min, Xiaojie¹  Wang, Mingwu²  Luo, Wenting³  Wu, Di¹  Yan, Qichang¹  Li, Jing¹  Wu, Xinwei¹  Zhang, Jinsong¹ 

[1] Fourth Affiliated Hosp China Med Univ, Eye Hosp China Med Univ, Dept Ophthalmol, Key Lens Res Lab Liaoning Prov, Shenyang 110005, Liaoning, Peoples R China

[2] Univ Arizona, Coll Med, Dept Ophthalmol & Vis Sci, Tucson, AZ USA

[3] China Med Univ, Shengjing Hosp, Key Lab Hlth Minist Congenital Malformat, Liaoning, Peoples R China

关键词: microRNA-125b;    p53;    Apoptosis;    Gene expression regulation;    Cataract;   

DOI  :  10.1016/j.bbadis.2014.10.002

来源: Elsevier

【 摘 要 】

MicroRNA-125b (miR-125b) has been implicated in a variety of diseases as either repressors or promoters, and plays crucial roles in many cellular processes such as cell differentiation, proliferation and apoptosis. Age-related cataract has become one of the most serious problems facing the aging population in the world. The purpose of this study was to investigate the role of miR-125b in the development of age-related cataract. We demonstrated that miR-125b was downregulated in both age-related cataract tissue and lens epithelial cell apoptosis induced by UV irradiation. We also identified the impact of miR-125b on apoptosis in a lens epithelial cell line. In vitro, miR-125b regulates human lens epithelial cell apoptosis at least in part by directly targeting p53. In addition, an inverse relationship between miR-125b and p53 expression was seen in age-related cataract tissue. In conclusion, this study suggests that miR-125b might be closely involved in the pathogenesis of cataract, and has the potential to be a diagnostic biomarker or even a therapeutic modality for cataract. (C) 2014 Elsevier B.V. All rights reserved.

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