Extraction and quantification of polyphosphates in activated sludge from waste water treatment plants by 31P NMR spectroscopy

WATER RESEARCH

卷:157

Article

Staal, Line Boisen¹  Petersen, Anne Boisen¹  Jorgensen, Charlotte A.¹  Nielsen, Ulla Gro²  Nielsen, Per Halkaejr³  Reitzel, Kasper¹ 

[1] Univ Southern Denmark, Dept Biol, Campusvej 55, DK-5230 Odense M, Denmark

[2] Univ Southern Denmark, Dept Phys Chem & Pharm, Campusvej 55, DK-5230 Odense M, Denmark

[3] Aalborg Univ, Ctr Microbial Communities, Dept Chem & Biosci, Fredrik Bajers Vej 7H, DK-9220 Aalborg, Denmark

关键词: Polyphosphate;    Identification;    Quantification;    P-31;    Solid state NMR;    EBPR;    Biological waste water treatment;   

DOI  :  10.1016/j.watres.2019.03.065

来源: Elsevier

【 摘 要 】

Polyphosphate (poly-P) is a major constituent in activated sludge from wastewater treatment plants with enhanced biological phosphorus removal due to poly-P synthesis by poly-P accumulating organisms where it plays an important role for recovery of phosphorus from waste water. Our aim was to develop a reliable protocol for poly-P quantification by P-31 NMR spectroscopy. This has so far been complicated by the risks of inefficient extraction and poly-P hydrolysis in the extracts. A protocol for complete extraction, identification and quantification of poly-P in activated sludge from a waste water treatment plant was identified based on test and evaluation of existing extraction protocols in combination with poly-P determination and quantification by solution and solid state P-31 NMR spectroscopy. The total poly-P middle group content was quantified by solid state NMR for comparison with the poly-P middle groups quantified by solution NMR, which is novel. Three different extraction protocols previously used in literature were compared: 1) a single 0.25 M NaOH-0.05 M EDTA extraction, 2) a 0.05 M EDTA pre-extraction followed by a 0.25 M NaOH main extraction and 3) a 0.05 M EDTA pre-extraction followed by a 0.25 M NaOH-0.05 M EDTA main extraction. The results showed that the extraction protocol 2 was optimal for fresh activated sludge, extracting 10.8 +/- 0.4 to 11.4 +/- 1.2 mgP/gDW poly-P. Extraction protocols 1 and 3 extracted less than 9.4 +/- 0.5 mgP/gDW poly-P. A comparison of the quantification of poly-P by P-31 solution NMR and by P-31 solid state NMR spectroscopy of lyophilised activated sludge showed 86 +/- 9% extraction efficiency of poly-P, which confirms that the extraction protocol recovered most of the poly-P from the samples without pronounced poly-P degradation. (C) 2019 Elsevier Ltd. All rights reserved.

【 授权许可】

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