Operation-driven heterogeneity and overlooked feed-associated populations in global anaerobic digester microbiome

WATER RESEARCH

卷:124

Article

Mei, Ran¹  Nobu, Masaru K.^1,2  Narihiro, Takashi²  Kuroda, Kyohei^1,3  Sierra, Julian Munoz⁴  Wu, Zhuoying⁵  Ye, Lin⁶  Lee, Patrick K. H.⁷  Lee, Po-Heng⁵  van Lier, Jules B.⁴  McInerney, Michael J.⁸  Kamagata, Yoichi²  Liu, Wen-Tso¹ 

[1] Univ Illinois, Dept Civil & Environm Engn, Urbana, IL 61801 USA

[2] Natl Inst Adv Ind Sci & Technol, Bioprod Res Inst, Tsukuba, Ibaraki, Japan

[3] Nagaoka Univ Technol, Dept Environm Syst Engn, Kami Tomioka, Niigata, Japan

[4] Delft Univ Technol, Dept Water Management, Sect Sanit Engn, Delft, Netherlands

[5] Hong Kong Polytech Univ, Dept Civil & Environm Engn, Kowloon, Hong Kong, Peoples R China

[6] Nanjing Univ, Sch Environm, Nanjing, Jiangsu, Peoples R China

[7] City Univ Hong Kong, Sch Energy & Environm, Kowloon, Hong Kong, Peoples R China

[8] Univ Oklahoma, Dept Bot & Microbiol, Norman, OK 73019 USA

关键词: Anaerobic digester;    Microbiome;    Operation;    Feed sludge;   

DOI  :  10.1016/j.watres.2017.07.050

来源: Elsevier

【 摘 要 】

Anaerobic digester (AD) microbiomes harbor complex, interacting microbial populations to achieve biomass reduction and biogas production, however how they are influenced by operating conditions and feed sludge microorganisms remain unclear. These were addressed by analyzing the microbial communities of 90 full-scale digesters at 51 municipal wastewater treatment plants from five countries. Heterogeneity detected in community structures suggested that no single AD microbiome could be defined. Instead, the AD microbiomes were classified into eight clusters driven by operating conditions (e.g., pretreatment, temperature range, and salinity), whereas geographic location of the digesters did not have significant impacts. Comparing digesters populations with those present in the corresponding feed sludge led to the identification of a hitherto overlooked feed-associated microbial group (i.e., the residue populations). They accounted for up to 21.4% of total sequences in ADs operated at low temperature, presumably due to ineffective digestion, and as low as 0.8% in ADs with pretreatment. Within each cluster, a core microbiome was defined, including methanogens, syntrophic metabolizers, fermenters, and the newly described residue populations. Our work provides insights into the key factors shaping full-scale AD microbiomes in a global scale, and draws attentions to the overlooked residue populations. (C) 2017 Elsevier Ltd. All rights reserved.

【 授权许可】

Free