Distinct growth stages controlled by the interplay of deterministic and stochastic processes in functional anammox biofilms

WATER RESEARCH

卷:200

Article

Niederdorfer, Robert¹  Fragner, Lisa¹  Yuan, Ling²  Hausherr, Damian³  Wei, Jing⁴  Magyar, Paul⁵  Joss, Adriano³  Lehmann, Moritz F.⁵  Ju, Feng²  Buergmann, Helmut¹ 

[1] Eawag, Swiss Fed Inst Aquat Sci & Technol, Dept Surface Waters Res & Management, CH-6047 Kastanienbaum, Switzerland

[2] Westlake Univ, Sch Engn, Key Lab Coastal Environm & Resources Zhejiang Pro, Hangzhou, Peoples R China

[3] Eawag, Swiss Fed Inst Aquat Sci & Technol, Dept Proc Engn, CH-8600 Dubendorf, Switzerland

[4] Empa, Swiss Fed Labs Mat Sci & Technol, Lab Air Pollut Environm & Technol, CH-8600 Dubendorf, Switzerland

[5] Univ Basel, Dept Environm Sci, Basel, Switzerland

关键词: mainstream anammox;    biofilm succession;    FISH;    amplicon sequencing;    sequencing;    batch reactor;    biofilm structure;   

DOI  :  10.1016/j.watres.2021.117225

来源: Elsevier

【 摘 要 】

Mainstream anaerobic ammonium oxidation (anammox) represents one of the most promising energyefficient mechanisms of fixed nitrogen elimination from wastewaters. However, little is known about the exact processes and drivers of microbial community assembly within the complex microbial biofilms that support anammox in engineered ecosystems. Here, we followed anammox biofilm development on fresh carriers in an established 8m 3 mainstream anammox reactor that is exposed to seasonal temperature changes (-25-12 degrees C) and varying NH 4 + concentrations (5-25 mg/L). We use fluorescence in situ hybridization and 16S rRNA gene sequencing to show that three distinct stages of biofilm development emerge naturally from microbial community composition and biofilm structure. Neutral modelling and network analysis are employed to elucidate the relative importance of stochastic versus deterministic processes and synergistic and antagonistic interactions in the biofilms during their development. We find that the different phases are characterized by a dynamic succession and an interplay of both stochastic and deterministic processes. The observed growth stages ( Colonization, Succession and Maturation ) appear to be the prerequisite for the anticipated growth of anammox bacteria and for reaching a biofilm community structure that supports the desired metabolic and functional capacities observed for biofilm carriers already present in the system (-100g NH4-N m 3 d -1 ). We discuss the relevance of this improved understanding of anammox-community ecology and biofilm development in the context of its practical application in the start-up, configuration, and optimization of anammox biofilm reactors. (c) 2021 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license ( http://creativecommons.org/licenses/by/4.0/ )

【 授权许可】

Free