Continuous ozonation of urban wastewater: Removal of antibiotics, antibiotic-resistant Escherichia coli and antibiotic resistance genes and phytotoxicity

WATER RESEARCH

卷:159

Article

Iakovides, I. C.^1,2  Michael-Kordatou, I.²  Moreira, N. F. F.^3,4  Ribeiro, A. R.⁴  Fernandes, T.⁵  Pereira, M. F. R.⁴  Nunes, O. C.³  Manaia, C. M.⁵  Silva, A. M. T.⁴  Fatta-Kassinos, D.^1,2 

[1] Univ Cyprus, Sch Engn, Dept Civil & Environm Engn, POB 20537, CY-1678 Nicosia, Cyprus

[2] Univ Cyprus, Nireas Int Water Res Ctr, POB 20537, CY-1678 Nicosia, Cyprus

[3] Univ Porto, Fac Engn, LEPABE Lab Proc Engn Environm Biotechnol & Energy, Rua Dr Roberto Frias, P-4200465 Porto, Portugal

[4] Univ Porto, LSRE, Fac Engn, LCM, Rua Dr Roberto Frias, P-4200465 Porto, Portugal

[5] Univ Catolica Portuguesa, CBQF, Lab Associado, Escola Super Biotecnol, Rua Arquiteto Lobao Vital 172, P-4200374 Porto, Portugal

关键词: Antibiotics;    Antibiotic resistance;    Phytotoxicity;    Ozonation;    Continuous mode;   

DOI  :  10.1016/j.watres.2019.05.025

来源: Elsevier

【 摘 要 】

This work evaluated the removal of a mixture of eight antibiotics (i.e. ampicillin (AMP), azithromycin (AZM), erythromycin (ERY), clarithromycin (CIA), ofloxacin (OFL), sulfamethoxazole (SMX), trimethoprim (TMP) and tetracycline (TC)) from urban wastewater, by ozonation operated in continuous mode at different hydraulic retention times (HRTs) (i.e. 10, 20, 40 and 60 min) and specific ozone doses (i.e. 0.125, 0.25, 0.50 and 0.75 gO(3) gDOC(-1)). As expected, the efficiency of ozonation was highly ozone dose-and contact time-dependent. The removal of the parent compounds of the selected antibiotics to levels below their detection limits was achieved with HRT of 40 min and specific ozone dose of 0.125 gO(3) gDOC(-1). The effect of ozonation was also investigated at a microbiological and genomic level, by studying the efficiency of the process with respect to the inactivation of Escherichia coli and antibiotic-resistant E. coli, as well as to the reduction of the abundance of selected antibiotic resistance genes (ARG5). The inactivation of total cultivable E. coli was achieved under the experimental conditions of HRT 40 min and 0.25 gO(3) gDOC(-1), at which all antibiotic compounds were already degraded. The regrowth examinations revealed that higher ozone concentrations were required for the permanent inactivation of E. coli below the Limit of Quantification (

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