Effect of conventional chemical treatment on the microbial population in a biofouling layer of reverse osmosis systems

WATER RESEARCH

卷:45

Article

Bereschenko, L. A.^1,2,3  Prummel, H.⁴  Euverink, G. J. W.¹  Stams, A. J. M.²  van Loosdrech, M. C. M.³ 

[1] Wetsus, Ctr Excellence Sustainable Water Technol, NL-8900 CC Leeuwarden, Netherlands

[2] Wageningen Univ, Microbiol Lab, NL-6703 HB Wageningen, Netherlands

[3] Delft Univ Technol, Dept Biotechnol, NL-2628 BC Delft, Netherlands

[4] Waterlab Noord, NL-9756 AD Delft, Netherlands

关键词: Biofilm;    Membrane;    Sphingomonas;    Clone library;    CSLM;    DGGE;   

DOI  :  10.1016/j.watres.2010.07.058

来源: Elsevier

【 摘 要 】

The impact of conventional chemical treatment on initiation and spatiotemporal development of biofilms on reverse osmosis (RO) membranes was investigated in situ using flow cells placed in parallel with the RO system of a full-scale water treatment plant. The flow cells got the same feed (extensively pre-treated fresh surface water) and operational conditions (temperature, pressure and membrane flux) as the full-scale installation. With regular intervals both the full-scale RO membrane modules and the flow cells were cleaned using conventional chemical treatment. For comparison some flow cells were not cleaned. Sampling was done at different time periods of flow cell operation (i.e., 1, 5, 10 and 17 days and 1, 3, 6 and 12 months). The combination of molecular (FISH, DGGE, clone libraries and sequencing) and microscopic (field emission scanning electron, epifluorescence and confocal laser scanning microscopy) techniques made it possible to thoroughly analyze the abundance, composition and 3D architecture of the emerged microbial layers. The results suggest that chemical treatment facilitates initiation and subsequent maturation of biofilm structures on the RO membrane and feed-side spacer surfaces. Biofouling control might be possible only if the cleaning procedures are adapted to effectively remove the (dead) biomass from the RO modules after chemical treatment. (c) 2010 Elsevier Ltd. All rights reserved.

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