Long-term monitoring of SARS-COV-2 RNA in wastewater in Brazil: A more responsive and economical approach

WATER RESEARCH

卷:203

Article

Mantovani Claro, Ieda Carolina¹  Cabral, Aline Diniz¹  Augusto, Matheus Ribeiro¹  Alves Duran, Adriana Feliciano¹  Pereira Graciosa, Melissa Cristina¹  Affonso Fonseca, Fernando Luiz³  Speranca, Marcia Aparecida²  Bueno, Rodrigo de Freitas¹ 

[1] Fed Univ ABC Ctr Engn, Modelling & Appl Social Sci CECS, Santo Andre, SP, Brazil

[2] Fed Univ ABC, Ctr Nat & Human Sci CCNH, Sao Bernardo, SP, Brazil

[3] Fac Med ABC FMABC, Dept Clin Anal, Santo Andre, SP, Brazil

关键词: COVID-19;    Wastewater-based epidemiology;    SARS-CoV-2;    Environmental surveillance;    Sewage;    Coronavirus;   

DOI  :  10.1016/j.watres.2021.117534

来源: Elsevier

【 摘 要 】

SARS-CoV-2, the novel Coronavirus, was first detected in Wuhan, China, in December 2019, and has since spread rapidly, causing millions of deaths worldwide. As in most countries of the world, in Brazil, the consequences of the COVID-19 pandemic have been catastrophic. Several studies have reported the fecal shedding of SARS-CoV-2 RNA titers from infected symptomatic and asymptomatic individuals. Therefore, the quantification of SARS-CoV-2 in wastewater can be used to track the virus spread in a population. In this study, samples of untreated wastewater were collected for 44 weeks at five sampling sites in the ABC Region (Sao Paulo, Brazil), in order to evaluate the SARS-CoV-2 occurrence in the sewerage system. SARS-CoV-2 RNA titers were detected throughout the period, and the concentration ranged from 2.7 to 7.7 log(10) genome copies.L-1, with peaks in the last weeks of monitoring. Furthermore, we observed a positive correlation between the viral load in wastewater and the epidemiological/clinical data, with the former preceding the latter by approximately two weeks. The COVID-19 prevalence for each sampling site was estimated via Monte-Carlo simulation using the wastewater viral load. The mean predicted prevalence ranged 0.05 to 0.38%, slightly higher than reported (0.016 +/- 0.005%) in the ABC Region for the same period. These results highlight the viability of the wastewater surveillance for COVID-19 infection monitoring in the largest urban agglomeration in South America. This approach can be especially useful for health agencies and public decision-makers in predicting SARS-CoV-2 outbreaks, as well as in local tracing of infection clusters.

【 授权许可】

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