Nutrients influence the dynamics of Klebsiella pneumoniae carbapenemase producing enterobacterales in transplanted hospital sinks

WATER RESEARCH

卷:176

Article

Kotay, Shireen Meher¹  Parikh, Hardik, I¹  Barry, Katie¹  Gweon, Hyun Soon³  Guilford, William²  Carroll, Joanne⁴  Mathers, Amy J.^1,4 

[1] Univ Virginia Hlth Syst, Dept Med, Div Infect Dis & Int Hlth, Charlottesville, VA USA

[2] Univ Virginia, Sch Med, Dept Biomed Engn, Charlottesville, VA 22908 USA

[3] Univ Reading, Sch Biol Sci, Reading, Berks, England

[4] Univ Virginia Hlth Syst, Dept Pathol, Clin Microbiol, Charlottesville, VA USA

关键词: Hospital sinks;    Drain;    P-trap;    Biofilms;    CPE;    Nutrients;    Microbiome;   

DOI  :  10.1016/j.watres.2020.115707

来源: Elsevier

【 摘 要 】

Antimicrobial resistance has been recognized as a threat to human health. The role of hospital sinks acting as a reservoir for some of the most concerning antibiotic resistant organisms, carbapenemase producing Enterobacterales (CPE) is evident but not well understood. Strategies to prevent establishment, interventions to eliminate these reservoirs and factors which drive persistence of CPE are not well established. We use a uniquely designed sink lab to transplant CPE colonized hospital sink plumbing with an aim to understand CPE dynamics in a controlled setting, notably exploiting both molecular and culture techniques. After ex situ installation the CPE population in the sink plumbing drop from previously detectable to undetectable levels. The addition of nutrients is followed by a quick rebound in CPE detection in the sinks after as many as 37 days. We did not however detect a significant shift in microbial community structure or the overall resistance gene carriage in longitudinal samples from a subset of these transplanted sinks using whole shotgun metagenomic sequencing. Comparing nutrient types in a benchtop culture study model, protein rich nutrients appear to be the most supportive for CPE growth and biofilm formation ability. The role of nutrients exposure is determining factor for maintaining a high bioburden of CPE in the sink drains and P-traps. Therefore, limiting nutrient disposal into sinks has reasonable potential with regard to decreasing the CPE wastewater burden, especially in hospitals seeking to control an environmental reservoir. (C) 2020 The Authors. Published by Elsevier Ltd.

【 授权许可】

Free