Transcriptomic approach for assessment of the impact on microalga and macrophyte of in-situ exposure in river sites contaminated by chlor-alkali plant effluents

WATER RESEARCH

卷:121

Article

Dranguet, Perrine¹  Cosio, Claudia¹  Le Faucheur, Severine¹  Beauvais-Flueck, Rebecca¹  Freiburghaus, Aline¹  Worms, Isabelle A. M.¹  Petit, Brice²  Civic, Natacha²  Docquier, Mylene²  Slaveykova, Vera I.¹ 

[1] Univ Geneva, Uni Carl Vogt, Fac Sci,Environm Biogeochem & Ecotoxicol, Dept FA Forel Environm & Aquat Sci Earth & Enviro, 66 Bvd Carl Vogt, CH-1211 Geneva 4, Switzerland

[2] Univ Geneva Med Sch CMU, iGE3 Genom Platform, 1 Rue Michel Servet, CH-1211 Geneva, Switzerland

关键词: Chlamydomonas reinhardtii;    Elodea nuttallii;    Environmental toxicity;    In-situ exposure;    Mercury;    NaCl;    Water quality;   

DOI  :  10.1016/j.watres.2017.05.020

来源: Elsevier

【 摘 要 】

Water quality degradation is a worldwide problem, but risk evaluation of chronic pollution in-situ is still a challenge. The present study aimed to evaluate the potential of transcriptomic analyses in representative aquatic primary producers to assess the impact of environmental pollution in-situ: the microalga Chlamydomonas reinhardtii and the macrophyte Elodea nuttallii were exposed 2 h in the Babeni Reservoir of the Olt River impacted by chlor-alkali plant effluent release resulting in increased concentrations of Hg and NaCl in receiving water. The response at the transcriptomic level was strong, resulting in up to 5485, and 8700 dysregulated genes (DG) for the microalga and for the macrophyte exposed in the most contaminated site, respectively. Transcriptomic response was congruent with the concentrations of Hg and NaCl in the water of the impacted reservoir. Genes involved in development, energy metabolism, lipid metabolism, nutrition, and RedOx homeostasis were dysregulated during in-situ exposure of both organisms. In addition, genes involved in the cell motility of C. reinhardtii and development of the cell wall of E. nuttallii were affected. DG were in line with adverse outcome pathways and transcriptomic studies reported after exposure to high concentrations of Hg and NaCl under controlled conditions in the laboratory. Transcriptomic response provided a sensitive measurement of the exposure as well as hints on the tolerance mechanisms of environmental pollution, and is thus promising as an early-warning tool to assess water quality degradation. (C) 2017 Elsevier Ltd. All rights reserved.

【 授权许可】

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