Antibiotic resistant Pseudomonas spp. in the aquatic environment: A prevalence study under tropical and temperate climate conditions

WATER RESEARCH

卷:115

Article

Devarajan, Naresh^1,2  Kohler, Thilo^3,4  Sivalingam, Periyasamy^1,2  van Delden, Christian^3,4  Mulaji, Crispin K.⁵  Mpiana, Pius T.⁵  Ibelings, Bastiaan W.^1,2  Pote, John^1,2,5 

[1] Univ Geneva, Fac Sci, Sect Earth & Environm Sci, Dept FA Forel Environm & Aquat Sci, Blvd Carl Vogt 66, CH-1205 Geneva, Switzerland

[2] Univ Geneva, Inst Environm Sci, Blvd Carl Vogt 66, CH-1205 Geneva, Switzerland

[3] Univ Geneva, Dept Microbiol & Mol Med, 1 Rue Michel Servet, CH-1211 Geneva 4, Switzerland

[4] Univ Hosp Geneva, Transplant Infect Dis Unit, 4 Rue Gabrielle Perret Gentil, CH-1211 Geneva 14, Switzerland

[5] Univ Kinshasa UNIKIN, Fac Sci, Dept Chem, BP 190, Kinshasa 11, DEM REP CONGO

关键词: Urban wastewater;    Aquatic environment contamination;    Extended spectrum beta-lactamases;    Pseudomonas species;    Antibiotic resistance;    Climatic condition;   

DOI  :  10.1016/j.watres.2017.02.058

来源: Elsevier

【 摘 要 】

Microbial populations which are resistant to antibiotics are an emerging environmental concern with potentially serious implications for public health. Thus, there is a growing concern in exploring the occurrence of antibiotic resistance in the environment with no limitations to the factors that contribute to their emergence. The aquatic environment is considered to be a hot-spot for the acquisition and spread of antibiotic resistance due to pollution with emerging contaminants derived from anthropogenic activities. In this study, we report on the isolation and characterization of 141 Pseudomonas spp. from aquatic sediments receiving partially (un)treated hospital and communal effluents from three distinct geographical locations: Democratic Republic of the Congo (DRC), India (IN), and Switzerland (CH). P. putida (42%) and P. aeruginosa (39%) were the dominant Pseudomonas species. The highest frequency of antibiotic resistance against eight anti-pseudomonas agents was found among IN isolates (35-60%), followed by DRC (18-50%) and CH (12-54%). CTX-M was the most frequent beta-lactamase found in CH (47% of isolates), while VIM-1 was dominant in isolates from DRC (61%) and IN (29%). NDM-1 was found in 29% of the total IN isolates and surprisingly also in 6% of CH isolates. Chromosomally-encoded efflux mechanisms were overexpressed in P. aeruginosa isolates from all three geographic locations. In vitro conjugative transfers of antibiotic resistance plasmids occurred more frequently under tropical temperatures (30 and 37 degrees C) than under temperate conditions (10 degrees C). The presence of Extended Spectrum beta-lactamases (ESBLs) and Metallo beta-lactamases (MBLs) in the isolates from environmental samples has important implications for humans who depend on public water supply and sanitation facilities. To our knowledge, this is the first study to demonstrate a comparison between treated/untreated effluents from urban and hospital settings as a source of microbial resistance by evaluating the aquatic ecosystems sediments from tropical and temperate climate conditions. Taken together, our findings demonstrate a widespread occurrence of antibiotic resistance in aquatic ecosystems sediments receiving untreated/treated wastewater and how these contemporary sources of contamination, contribute to the spread of microbial resistance in the aquatic environment. This research presents also useful tools to evaluate sediment quality in the receiving river/reservoir systems which can be applied to similar environments. (C) 2017 Elsevier Ltd. All rights reserved.

【 授权许可】

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