Isolation of TNT tolerant pseudomonas species (Strain KA) from TNT contaminated soil biotransformation of TNT

Defence Science Journal

Avinash M. Tope¹  Kaiser Jamil¹ 

[1] Indian Institute of Chemical Technology, Hyderabad

关键词: Biotransformation;    TNT metabolism;    microbial degradation;    Pseudomonas species;   

DOI  :  

学科分类：社会科学、人文和艺术（综合）

来源: Defence Scientific Information & Documentation Centre

【 摘 要 】

"A 2,4,6-trinitrotoluene (TNT) utilisingbacterium, Pseudomonas species (strain KA) was isolated from soils of amunitions processing unit and studied for itsability to grow andmetabolise TNT. The result indicated that the isolate could growaerobically in a minimal salt medium containing 0.25 mM/l TNT at 30 °C.It could completely transform 0.25 mM/l TNT in 5 days giving 2-isomericmonoaminodinitrotoluenes, namely 4-aminodinitrotoluene and2-aminodinitrotoluene. Products of TNT transformation were analysed andconfirmed by thin layer chromatography and gas chromatography coupledwith mass spectrometry. Both, growth of the isolate andbiotransformation rates were better supported on sugar and ammonium saltwhen added to the medium separately. Pseudomonas species (strain KA)showed maximum TNT transformation efficiency in the presence of mannose.For degradation of TNT, this strain can be employed to initiate theprocess in association with the other microbial members that cancollectively degrade TNT .

【 授权许可】

Unknown