期刊论文详细信息
Frontiers in Plant Science
An artificial intelligence-integrated analysis of the effect of drought stress on root traits of “modern” and “ancient” wheat varieties
Plant Science
Clarissa Zanotti1  Anna Fiorillo1  Mauro Marra1  Domenico Felice2  Ilva Licaj3  Mariapina Rocco3  Chiara Germinario3 
[1] Department of Biology, University of Tor Vergata, Rome, Italy;Department of Management Engineering, Polytechnic of Milan, Milan, Italy;Department of Science and Technology, University of Sannio, Benevento, Italy;
关键词: wheat;    osmotic stress;    root;    artificial intelligence;    phytohormones;   
DOI  :  10.3389/fpls.2023.1241281
 received in 2023-06-16, accepted in 2023-09-25,  发布年份 2023
来源: Frontiers
PDF
【 摘 要 】

Due to drought stress, durum wheat production in the Mediterranean basin will be severely affected in the coming years. Durum wheat cultivation relies on a few genetically uniform "modern" varieties, more productive but less tolerant to stresses, and "traditional" varieties, still representing a source of genetic biodiversity for drought tolerance. Root architecture plasticity is crucial for plant adaptation to drought stress and the relationship linking root structures to drought is complex and still largely under-explored. In this study, we examined the effect of drought stress on the roots’ characteristics of the “traditional” Saragolla cultivar and the “modern” Svevo. By means of “SmartRoot” software, we demonstrated that drought stress affected primary and lateral roots as well as root hair at different extents in Saragolla and Svevo cultivars. Indeed, we observed that under drought stress Saragolla possibly revamped its root architecture, by significantly increasing the length of lateral roots, and the length/density of root hairs compared to the Svevo cultivar. Scanning Electron Microscopy analysis of root anatomical traits demonstrated that under drought stress a greater stele area and an increase of the xylem lumen size vessel occurred in Saragolla, indicating that the Saragolla variety had a more efficient adaptive response to osmotic stress than the Svevo. Furthermore, for the analysis of root structural data, Artificial Intelligence (AI) algorithms have been used: Their application allowed to predict from root structural traits modified by the osmotic stress the type of cultivar observed and to infer the relationship stress-cultivar type, thus demonstrating that root structural traits are clear and incontrovertible indicators of the higher tolerance to osmotic stress of the Saragolla cultivar. Finally, to obtain an integrated view of root morphogenesis, phytohormone levels were investigated. According to the phenotypic effects, under drought stress,a larger increase in IAA and ABA levels, as well as a more pronounced reduction in GA levels occurred in Saragolla as compared to Svevo. In conclusion, these results show that the root growth and hormonal profile of Saragolla are less affected by osmotic stress than those of Svevo, demonstrating the great potential of ancient varieties as reservoirs of genetic variability for improving crop responses to environmental stresses.

【 授权许可】

Unknown   
Copyright © 2023 Licaj, Felice, Germinario, Zanotti, Fiorillo, Marra and Rocco

【 预 览 】
附件列表
Files Size Format View
RO202311148863944ZK.pdf 5493KB PDF download
  文献评价指标  
  下载次数:1次 浏览次数:0次