THROUGH THE LOOKING GLASS: Real-Time Imaging in Brachypodium Roots and Osmotic Stress Analysis

Plants

Hikmet Budak¹  Hande Karamahmutoğlu²  Meltem Elitaş²  Zaeema Khan³  Meral Yüce⁴ 

[1] Cereal Genomics Lab, Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT 59717, USA;Mechatronics Program, Faculty of Engineering and Natural Sciences, Sabanci University, Istanbul 34956, Turkey;Molecular Biology, Genetics and Bioengineering Program, Faculty of Engineering and Natural Sciences, Sabanci University, Istanbul 34956, Turkey;Sabanci University SUNUM Nanotechnology Research and Application Centre, Istanbul 34956, Turkey;

关键词: Brachypodium;    neutral red;    root;    Casparian bands;    PEG-6000;    osmotic stress;    real-time imaging;    PDMS;   

DOI  :  10.3390/plants8010014

来源: DOAJ

【 摘 要 】

To elucidate dynamic developmental processes in plants, live tissues and organs must be visualised frequently and for extended periods. The development of roots is studied at a cellular resolution not only to comprehend the basic processes fundamental to maintenance and pattern formation but also study stress tolerance adaptation in plants. Despite technological advancements, maintaining continuous access to samples and simultaneously preserving their morphological structures and physiological conditions without causing damage presents hindrances in the measurement, visualisation and analyses of growing organs including plant roots. We propose a preliminary system which integrates the optical real-time visualisation through light microscopy with a liquid culture which enables us to image at the tissue and cellular level horizontally growing Brachypodium roots every few minutes and up to 24 h. We describe a simple setup which can be used to track the growth of the root as it grows including the root tip growth and osmotic stress dynamics. We demonstrate the system’s capability to scale down the PEG-mediated osmotic stress analysis and collected data on gene expression under osmotic stress.

【 授权许可】

Unknown