Rational design of the genetic code expansion toolkit for in vivo encoding of D-amino acids

Frontiers in Genetics

Genetics

Dieter Söll¹  Jui-Hung Weng²  An-Li Andrea Ko²  Jo-Chu Tsou²  Ming-Daw Tsai²  Yi-Hui Wang³  Pei-Jung Chen³  Han-Kai Jiang⁴  Yane-Shih Wang⁵ 

[1] Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, United States;Department of Chemistry, Yale University, New Haven, CT, United States;Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan;Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan;Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan;Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan;Taiwan International Graduate Program Chemical Biology and Molecular Biophysics, Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan;Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan;Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan;Taiwan International Graduate Program Chemical Biology and Molecular Biophysics, Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan;Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan;

关键词: pyrrolysyl-tRNA synthetase;    genetic code expansion;    noncanonical amino acids;    D-phenylalanine analogs;    synthetic biology;    amber suppression;   

DOI  :  10.3389/fgene.2023.1277489

 received in 2023-08-14, accepted in 2023-09-18,  发布年份 2023

来源: Frontiers

【 摘 要 】

Once thought to be non-naturally occurring, D-amino acids (DAAs) have in recent years been revealed to play a wide range of physiological roles across the tree of life, including in human systems. Synthetic biologists have since exploited DAAs’ unique biophysical properties to generate peptides and proteins with novel or enhanced functions. However, while peptides and small proteins containing DAAs can be efficiently prepared in vitro, producing large-sized heterochiral proteins poses as a major challenge mainly due to absence of pre-existing DAA translational machinery and presence of endogenous chiral discriminators. Based on our previous work demonstrating pyrrolysyl-tRNA synthetase’s (PylRS’) remarkable substrate polyspecificity, this work attempts to increase PylRS’ ability in directly charging tRNAPyl with D-phenylalanine analogs (DFAs). We here report a novel, polyspecific Methanosarcina mazei PylRS mutant, DFRS2, capable of incorporating DFAs into proteins via ribosomal synthesis in vivo. To validate its utility, in vivo translational DAA substitution were performed in superfolder green fluorescent protein and human heavy chain ferritin, successfully altering both proteins’ physiochemical properties. Furthermore, aminoacylation kinetic assays further demonstrated aminoacylation of DFAs by DFRS2 in vitro.

【 授权许可】

Unknown   
Copyright © 2023 Jiang, Weng, Wang, Tsou, Chen, Ko, Söll, Tsai and Wang.