The effect of multiple outgrowths from bronchial tissue explants on progenitor/stem cell number in primary bronchial epithelial cell cultures from smokers and patients with COPD

Frontiers in Medicine

Medicine

Hasan Bayram¹  Seval Kul²  Ayhan Eralp³  Mehmet Yuncu³  Nuray Bostancieri⁴  Kemal Bakir⁵  Ozgecan Kayalar⁶  Nur Konyalilar⁶  Hadi Rajabi⁶  Ali Önder Yildirim⁷ 

[1] Cell Culture Laboratory, Department of Chest Diseases, School of Medicine, University of Gaziantep, Gaziantep, Türkiye;Koc University Research Center for Translational Medicine, Koc University, Istanbul, Türkiye;Department of Biostatistics, School of Medicine, University of Gaziantep, Gaziantep, Türkiye;Department of Histology and Embryology, School of Medicine, University of Gaziantep, Gaziantep, Türkiye;Department of Histology and Embryology, School of Medicine, University of Gaziantep, Gaziantep, Türkiye;Cell Culture Laboratory, Department of Chest Diseases, School of Medicine, University of Gaziantep, Gaziantep, Türkiye;Department of Pathology, School of Medicine, University of Gaziantep, Gaziantep, Türkiye;Koc University Research Center for Translational Medicine, Koc University, Istanbul, Türkiye;Koc University Research Center for Translational Medicine, Koc University, Istanbul, Türkiye;Comprehensive Pneumology Center (CPC), Institute of Lung Health and Immunity (LHI), Member of the German Center for Lung Research (DZL), Helmholtz Munich, Munich, Germany;

关键词: COPD;    smoker;    progenitor/stem cell;    primary bronchial epithelial cell culture;    cytokeratin 5;    cytokeratin 14;    p63;   

DOI  :  10.3389/fmed.2023.1118715

 received in 2022-12-07, accepted in 2023-09-25,  发布年份 2023

来源: Frontiers

【 摘 要 】

BackgroundAlthough studies suggest a deficiency in stem cell numbers in chronic airway diseases such as chronic obstructive pulmonary disease (COPD), the role of bronchial epithelial progenitor/stem (P/S) cells is not clear. The objectives of this study were to investigate expression of progenitor/stem (P/S) cell markers, cytokeratin (CK) 5, CK14 and p63 in bronchial epithelial explants and cell cultures obtained from smokers with and without COPD following multiple outgrowths, and to study this effect on bronchial epithelial cell (BEC) proliferation.MethodsBronchial epithelial explants were dissected from lung explants and cultured on coverslips. Confluent cultures were obtained after 3–4 weeks’ (transfer, Tr1), explants were then transferred and cultured for a second (Tr2) and third (Tr3) time, respectively. At each stage, expression of CK5, CK14 and p63 in explants and BEC were determined by immunostaining. In parallel experiments, outgrowing cells from explants were counted after 4wks, and explants subsequently transferred to obtain new cultures for a further 3 times.ResultsAs the transfer number advanced, CK5, CK14 and p63 expression was decreased in both explants and BEC from both smokers without COPD and patients with COPD, with a more pronounced decrease in BEC numbers in the COPD group. Total cell numbers cultured from explants were decreased with advancing outgrowth number in both groups. Smoking status and lung function parameters were correlated with reduced P/S marker expression and cell numbers.ConclusionOur findings suggest that the number of P/S cells in airway epithelium may play a role in the pathogenesis of COPD, as well as a role in the proliferation of airway epithelial cells, in vitro.

【 授权许可】

Unknown   
Copyright © 2023 Bostancieri, Bakir, Kul, Eralp, Kayalar, Konyalilar, Rajabi, Yuncu, Yildirim and Bayram.