Early life stress induces irritable bowel syndrome from childhood to adulthood in mice

Frontiers in Microbiology

Microbiology

Marong Fang¹  Zhenya Zhu²  Xiaoli Shu²  Ting Zhang²  Diya Ye²  Gao Long²  Bo Chen²  Chenmin Hu²  Rui Guo²  Yuhao Wu²  Xinyi Jia³  Xiao Du³  Mizu Jiang³  Wei Zheng³  Enfu Tao⁴ 

[1] Institute of Neuroscience and Gastrointestinal Laboratory, Children’s Hospital, Zhejiang University School of Medicine, Hangzhou, China;Pediatric Endoscopy Center and Gastrointestinal Laboratory, Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children's Regional Medical Center, Hangzhou, China;Pediatric Endoscopy Center and Gastrointestinal Laboratory, Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children's Regional Medical Center, Hangzhou, China;Department of Gastroenterology, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children's Regional Medical Center, Hangzhou, China;Pediatric Endoscopy Center and Gastrointestinal Laboratory, Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children's Regional Medical Center, Hangzhou, China;Department of Neonatology and NICU, Wenling Maternal and Child Health Care Hospital, Wenling, China;

关键词: irritable bowel syndrome;    early life stress;    brain-gut-microbiota axis;    maternal separation;    visceral hypersensitivity;   

DOI  :  10.3389/fmicb.2023.1255525

 received in 2023-07-10, accepted in 2023-09-15,  发布年份 2023

来源: Frontiers

【 摘 要 】

BackgroundIrritable bowel syndrome (IBS) is one of the most common functional gastrointestinal disorder. Traditionally, early life stress (ELS) is predisposed to IBS in adult. However, whether ELS induces IBS in early life remains unclear.MethodsSeparated cohort studies were conducted in neonatal male pups of C57BL/6 mice by maternal separation (MS) model. MS and non-separation mice were scheduled to be evaluated for prime IBS-phenotypes, including visceral hypersensitivity, intestinal motility, intestinal permeability, and anxiety-like behavior. Ileal contents and fecal samples were collected and analyzed by 16S rRNA gene sequencing and bacterial community analyses. Subcellular structures of intestinal epithelial, such as epithelial tight junctions and mitochondria, were observed under transmission electron microscopy.ResultsMS induced visceral hypersensitivity and decreased total intestinal transit time from childhood to adulthood. In addition, MS induced intestinal hyperpermeability and anxiety-like behavior from adolescence to adulthood. Besides, MS affected intestinal microbial composition from childhood to adulthood. Moreover, MS disrupted intestinal mitochondrial structure from childhood to adulthood.ConclusionThe study showed for the first time that MS induced IBS from early life to adulthood in mice. The disrupted intestinal mitochondrial structure and the significant dysbiosis of intestinal microbiota in early life may contribute to the initiation and progress of IBS from early life to adulthood.

【 授权许可】

Unknown   
Copyright © 2023 Tao, Wu, Hu, Zhu, Ye, Long, Chen, Guo, Shu, Zheng, Zhang, Jia, Du, Fang and Jiang.