Bone marrow stromal cells in Modic type 1 changes promote neurite outgrowth

Frontiers in Cell and Developmental Biology

Cell and Developmental Biology

Oliver Distler¹  Irina Heggli²  Nick Herger²  Tamara Mengis²  Jan Devan²  Stefan Dudli²  José Miguel Spirig³  Christoph J. Laux³  Mazda Farshad³  Florian Brunner⁴ 

[1] Center of Experimental Rheumatology, Department of Rheumatology, University Hospital Zurich, University of Zurich, Zürich, Switzerland;Center of Experimental Rheumatology, Department of Rheumatology, University Hospital Zurich, University of Zurich, Zürich, Switzerland;Department of Physical Medicine and Rheumatology, Balgrist University Hospital, University of Zurich, Zürich, Switzerland;Department of Orthopedics, Balgrist University Hospital, University of Zurich, Zürich, Switzerland;Department of Physical Medicine and Rheumatology, Balgrist University Hospital, University of Zurich, Zürich, Switzerland;

关键词: low back pain;    neurotrophic;    stromal cells;    Modic changes;    neurite outgrowth;    basivertebral nerve;   

DOI  :  10.3389/fcell.2023.1286280

 received in 2023-08-31, accepted in 2023-10-10,  发布年份 2023

来源: Frontiers

【 摘 要 】

The pain in patients with Modic type 1 changes (MC1) is often due to vertebral body endplate pain, which is linked to abnormal neurite outgrowth in the vertebral body and adjacent endplate. The aim of this study was to understand the role of MC1 bone marrow stromal cells (BMSCs) in neurite outgrowth. BMSCs can produce neurotrophic factors, which have been shown to be pro-fibrotic in MC1, and expand in the perivascular space where sensory vertebral nerves are located. The study involved the exploration of the BMSC transcriptome in MC1, co-culture of MC1 BMSCs with the neuroblastoma cell line SH-SY5Y, analysis of supernatant cytokines, and analysis of gene expression changes in co-cultured SH-SY5Y. Transcriptomic analysis revealed upregulated brain-derived neurotrophic factor (BDNF) signaling-related pathways. Co-cultures of MC1 BMSCs with SH-SY5Y cells resulted in increased neurite sprouting compared to co-cultures with control BMSCs. The concentration of BDNF and other cytokines supporting neuron growth was increased in MC1 vs. control BMSC co-culture supernatants. Taken together, these findings show that MC1 BMSCs provide strong pro-neurotrophic cues to nearby neurons and could be a relevant disease-modifying treatment target.

【 授权许可】

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Copyright © 2023 Mengis, Herger, Heggli, Devan, Spirig, Laux, Brunner, Farshad, Distler and Dudli.