Construction of an m6A- and neutrophil extracellular traps-related lncRNA model to predict hepatocellular carcinoma prognosis and immune landscape

Frontiers in Immunology

Immunology

Wei Wang¹  Na Lu²  Xiao Guan²  Tian Zhan²  Jianping Zhang²  Wei Bao³ 

[1] Department of Clinical Laboratory, Lianshui County People’s Hospital, Huai’an, China;Department of General Surgery, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China;Department of Urology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China;

关键词: long non-coding RNA;    N6-methyladenosine;    neutrophil extracellular traps;    hepatocellular carcinoma;    bioinformatics;   

DOI  :  10.3389/fimmu.2023.1231543

 received in 2023-05-30, accepted in 2023-09-21,  发布年份 2023

来源: Frontiers

【 摘 要 】

PurposeTo investigate the impact of N6-methyladenosine- (m6A) and neutrophil extracellular traps- (NETs) related lncRNAs (MNlncRNAs) on the prognosis of hepatocellular carcinoma (HCC).MethodsWe collected m6A and NETs-related genes from published studies. We identified the MNlncRNAs by correlation analysis. Cox regression and the least absolute selection operator (LASSO) method were used to select predictive MNlncRNAs. The expressions of predictive MNlncRNAs were detected by cell and tissue experiments. Survival, medication sensitivity, and immunological microenvironment evaluations were used to assess the model’s prognostic utility. Finally, we performed cellular experiments to further validate the model’s prognostic reliability.ResultsWe obtained a total of 209 MNlncRNAs. 7 MNlncRNAs comprised the prognostic model, which successfully stratifies HCC patients, with the area under the curve (AUC) ranging from 0.7 to 0.8. In vitro tests confirmed that higher risk patients had worse prognosis. Risk score, immunological microenvironment, and immune checkpoint gene expression were all significantly correlated with each other in HCC. In the group at high risk, immunotherapy could be more successful. Cellular assays confirmed that HCC cells with high risk scores have a higher proliferation and invasive capacity.ConclusionThe MNlncRNAs-related prognostic model aided in determining HCC prognosis, revealing novel therapeutic options, notably immunotherapy.

【 授权许可】

Unknown   
Copyright © 2023 Zhan, Wang, Guan, Bao, Lu and Zhang