Systems and synthetic biology-driven engineering of live bacterial therapeutics

Frontiers in Bioengineering and Biotechnology

Bioengineering and Biotechnology

Minjeong Kang¹  Kangsan Kim¹  Byung-Kwan Cho² 

[1] Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea;KAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea;Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea;KAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea;Graduate School of Engineering Biology, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea;

关键词: live biotherapeutics;    probiotics;    microbiome;    synthetic biology;    systems biology;   

DOI  :  10.3389/fbioe.2023.1267378

 received in 2023-07-26, accepted in 2023-10-09,  发布年份 2023

来源: Frontiers

【 摘 要 】

The past decade has seen growing interest in bacterial engineering for therapeutically relevant applications. While early efforts focused on repurposing genetically tractable model strains, such as Escherichia coli, engineering gut commensals is gaining traction owing to their innate capacity to survive and stably propagate in the intestine for an extended duration. Although limited genetic tractability has been a major roadblock, recent advances in systems and synthetic biology have unlocked our ability to effectively harness native gut commensals for therapeutic and diagnostic purposes, ranging from the rational design of synthetic microbial consortia to the construction of synthetic cells that execute “sense-and-respond” logic operations that allow real-time detection and therapeutic payload delivery in response to specific signals in the intestine. In this review, we outline the current progress and latest updates on microbial therapeutics, with particular emphasis on gut commensal engineering driven by synthetic biology and systems understanding of their molecular phenotypes. Finally, the challenges and prospects of engineering gut commensals for therapeutic applications are discussed.

【 授权许可】

Unknown   
Copyright © 2023 Kim, Kang and Cho.