Preliminary evaluation of the proteomic profiling in the hippocampus of aged grazing cattle

Frontiers in Aging Neuroscience

Aging Neuroscience

Maria Claudia Gatto¹  Maria Monti²  Flora Cozzolino²  Ilaria Iacobucci²  Luisa Canè³  Francesco Napolitano⁴  Davide De Biase⁵  Luigi Sacchettino⁶  Orlando Paciello⁶  Luigi Avallone⁶  Claudia Gatta⁶  Danila d’Angelo⁶  Evaristo Di Napoli⁶ 

[1] CEINGE-Biotecnologie Avanzate “Franco Salvatore”-Via G. Salvatore, Naples, Italy;CEINGE-Biotecnologie Avanzate “Franco Salvatore”-Via G. Salvatore, Naples, Italy;Department of Chemical Sciences, University of Naples, Federico II, Naples, Italy;CEINGE-Biotecnologie Avanzate “Franco Salvatore”-Via G. Salvatore, Naples, Italy;Department of Translational Medical Sciences, University of Naples Federico II, Naples, Italy;CEINGE-Biotecnologie Avanzate “Franco Salvatore”-Via G. Salvatore, Naples, Italy;Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy;Department of Pharmacy, University of Salerno, Fisciano, Italy;Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy;

关键词: aging;    bovine;    brain;    hippocampus;    proteomics;   

DOI  :  10.3389/fnagi.2023.1274073

 received in 2023-08-07, accepted in 2023-10-11,  发布年份 2023

来源: Frontiers

【 摘 要 】

Brain aging is a physiological process associated with physical and cognitive decline; however, in both humans and animals, it can be regarded as a risk factor for neurodegenerative disorders, such as Alzheimer’s disease. Among several brain regions, hippocampus appears to be more susceptible to detrimental effects of aging. Hippocampus belongs to limbic system and is mainly involved in declarative memories and context-dependent spatial-learning, whose integrity is compromised in an age-dependent manner. In the present work, taking advantage of liquid chromatography–tandem mass spectrometry (LC–MS/MS)-based proteomics, we sought to identify proteins differentially expressed in the hippocampus of the aged grazing milk cows. Our exploratory findings showed that, out of 707 identified proteins, 112 were significantly altered in old cattle, when compared to the adult controls, and functional clusterization highlighted their involvement in myelination, synaptic vesicle, metabolism, and calcium-related biological pathways. Overall, our preliminary data pave the way for the future studies, aimed at better characterizing the role of such a subcortical brain region in the age-dependent cognitive decline, as well as identifying early aging markers to improve animal welfare and husbandry practices of dairy cattle from intensive livestock.

【 授权许可】

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Copyright © 2023 Cozzolino, Canè, Sacchettino, Gatto, Iacobucci, Gatta, De Biase, Di Napoli, Paciello, Avallone, Monti, d’Angelo and Napolitano.