Characterization and fungicide sensitivity of Trichoderma species causing green mold of Ganoderma sichuanense in China

Frontiers in Microbiology

Microbiology

Qian Wei¹  Michael Wiafe-Kwagyan²  Changtian Li³  Frederick Leo Sossah⁴  Na Rong⁵  Shiyu Li⁵  Jiajun Hu⁶  Yonglan Tuo⁷  Bo Zhang⁷  Yu Li⁷  Xuefei Li⁷  Xiao Li⁷ 

[1] College of Plant Protection, Jilin Agricultural University, Changchun, China;Department of Plant and Environmental Biology, School of Biological Sciences, College of Basic and Applied Sciences, University of Ghana, Accra, Ghana;Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, China;Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, China;Coconut Research Programme, Council for Scientific and Industrial Research (CSIR), Oil Palm Research Institute, Kade, Ghana;Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, China;College of Plant Protection, Jilin Agricultural University, Changchun, China;Joint International Research Laboratory of Modern Agricultural Technology, Ministry of Education, Jilin Agricultural University, Changchun, China;Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, China;Joint International Research Laboratory of Modern Agricultural Technology, Ministry of Education, Jilin Agricultural University, Changchun, China;Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, China;College of Plant Protection, Jilin Agricultural University, Changchun, China;

关键词: Trichoderma;    Ganoderma sichuansense;    green mold disease;    pathogenicity;    fungicides;    prochloraz manganese;    mushroom health;   

DOI  :  10.3389/fmicb.2023.1264699

 received in 2023-07-21, accepted in 2023-09-28,  发布年份 2023

来源: Frontiers

【 摘 要 】

Green mold disease, caused by Trichoderma spp., is one of the most devastating diseases of mushrooms in China. The application of fungicides remains one of the important control methods among the integrated pest management tools for disease management in mushroom farms. This study aimed to identify Trichoderma spp., isolated from G. sichuanense fruiting bodies displaying green mold symptoms collected from mushroom farms in Zhejiang, Hubei, and Jilin Province, China, and evaluate their in vitro sensitivity to six fungicides. A total of 47 isolates were obtained and classified into nine Trichoderma spp. namely, T. asperellum, T. citrinoviride, T. ganodermatiderum, T. guizhouense, T. hamatum, T. harzianum, T. koningiopsis, T. paratroviride, and T. virens, through morphological characteristics and phylogenetic analysis of concatenated sequences of translation elongation factor 1-alpha (TEF) and DNA-dependent RNA polymerase II subunit (RPB2) genes. The pathogenicity test was repeated two times, and re-isolation of the nine Trichoderma spp. from the fruiting bodies of G. sichuanense fulfilled Koch’s postulates. Prochloraz manganese showed the best performance against most species. This research contributes to our understanding of green mold disease, reveals the phylogenetic relationships among Trichoderma species, and expands our knowledge of Trichoderma species diversity associated with green mold disease in G. sichuanense.

【 授权许可】

Unknown   
Copyright © 2023 Li, Sossah, Tuo, Hu, Wei, Li, Rong, Wiafe-Kwagyan, Li, Zhang, Li and Li.