Genetic characterization of root architectural traits in barley (Hordeum vulgare L.) using SNP markers

Frontiers in Plant Science

Plant Science

Art J. Diggle¹  Guihua Bai²  Amy Bernardo²  Paul St. Amand²  David Moody³  Zed Rengel⁴  M. Q. U. Farooqi⁴ 

[1] Department of Primary Industries and Regional Development, South Perth, WA, Australia;Hard Winter Wheat Genetics Research Unit, USDA-ARS, Manhattan, KS, United States;InterGrain, Bibra Lake, WA, Australia;UWA School of Agriculture and Environment, The University of Western Australia, Perth, WA, Australia;

关键词: barley;    genotyping-by-sequencing;    SNP markers;    association mapping;    root traits;   

DOI  :  10.3389/fpls.2023.1265925

 received in 2023-07-24, accepted in 2023-09-05,  发布年份 2023

来源: Frontiers

【 摘 要 】

Increasing attention is paid to providing new tools to breeders for targeted breeding for specific root traits that are beneficial in low-fertility, drying soils; however, such information is not available for barley (Hordeum vulgare L.). A panel of 191 barley accessions (originating from Australia, Europe, and Africa) was phenotyped for 26 root and shoot traits using the semi-hydroponic system and genotyped using 21 062 high-quality single nucleotide polymorphism (SNP) markers generated by genotyping-by-sequencing (GBS). The population structure analysis of the barley panel identified six distinct groups. We detected 1199 significant (P<0.001) marker-trait associations (MTAs) with r2 values up to 0.41. The strongest MTAs were found for root diameter in the top 20 cm and the longest root length. Based on the physical locations of these MTAs in the barley reference genome, we identified 37 putative QTLs for the root traits, and three QTLs for shoot traits, with nine QTLs located in the same physical regions. The genomic region 640-653 Mb on chromosome 7H was significant for five root length-related traits, where 440 annotated genes were located. The putative QTLs for various root traits identified in this study may be useful for genetic improvement regarding the adaptation of new barley cultivars to suboptimal environments and abiotic stresses.

【 授权许可】

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Copyright © 2023 Farooqi, Moody, Bai, Bernardo, St. Amand, Diggle and Rengel