Genotyping-by-Sequencing in Plants

Biology

Stéphane Deschamps¹  Victor Llaca¹ 

[1] DuPont Agricultural Biotechnology, Experimental Station, PO Box 80353, 200 Powder Mill Road, Wilmington, DE 19880-0353, USA;

关键词: genotyping;    genotyping-by-sequencing;    sequencing;    next-generation sequencing;    genome-wide association;    DNA;    SNPs;    NGS;    GBS;   

DOI  :  10.3390/biology1030460

来源: mdpi

【 摘 要 】

The advent of next-generation DNA sequencing (NGS) technologies has led to the development of rapid genome-wide Single Nucleotide Polymorphism (SNP) detection applications in various plant species. Recent improvements in sequencing throughput combined with an overall decrease in costs per gigabase of sequence is allowing NGS to be applied to not only the evaluation of small subsets of parental inbred lines, but also the mapping and characterization of traits of interest in much larger populations. Such an approach, where sequences are used simultaneously to detect and score SNPs, therefore bypassing the entire marker assay development stage, is known as genotyping-by-sequencing (GBS). This review will summarize the current state of GBS in plants and the promises it holds as a genome-wide genotyping application.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.