Regulation of astrocyte activity and immune response on graphene oxide-coated titanium by electrophoretic deposition

Frontiers in Bioengineering and Biotechnology

Bioengineering and Biotechnology

Yong-Soo Han¹  Won-Seok Lee²  Bo-Eun Yoon³  Jun-Sung Oh⁴  Jun-Hwee Jang⁴  Eun-Jung Lee⁴ 

[1] Department of Molecular Biology, College of Science and Technology, Dankook University, Cheonan, Republic of Korea;Department of Molecular Biology, College of Science and Technology, Dankook University, Cheonan, Republic of Korea;Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Republic of Korea;Mechanobiology Dental Medicine Research Center, Dankook University, Cheonan, Republic of Korea;Department of Molecular Biology, College of Science and Technology, Dankook University, Cheonan, Republic of Korea;Nano-Bio Medical Science, Graduate School, Dankook University, Cheonan, Republic of Korea;Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Republic of Korea;Mechanobiology Dental Medicine Research Center, Dankook University, Cheonan, Republic of Korea;Nano-Bio Medical Science, Graduate School, Dankook University, Cheonan, Republic of Korea;

关键词: astrocyte;    anti-inflammatory cytokine;    electrophoretic deposition;    graphene oxide;    titanium;   

DOI  :  10.3389/fbioe.2023.1261255

 received in 2023-07-19, accepted in 2023-09-19,  发布年份 2023

来源: Frontiers

【 摘 要 】

Introduction: Astrocytes play crucial role in modulating immune response in the damaged central nervous system. Numerous studies have investigated the relationship between immune responses in astrocytes and brain diseases. However, the potential application of nanomaterials for alleviating neuroinflammation induced by astrocytes remains unexplored.Method: In this study, we utilized electrophoretic deposition (EPD) to coat graphene oxide (GO) onto titanium (Ti) to enhance the bioactivity of Ti.Results: We confirmed that GO-Ti could improve cell adhesion and proliferation of astrocytes with upregulated integrins and glial fibrillary acidic protein (GFAP) expression. Moreover, we observed that astrocytes on GO-Ti exhibited a heightened immune response when exposed to lipopolysaccharide (LPS). Although pro-inflammatory cytokines increased, anti-inflammatory cytokines and brain-derived neurotrophic factors involved in neuroprotective effects were also augmented through nuclear localization of the yes-associated protein (YAP) and nuclear factor kappa B (NF‐κB).Discussion: Taken together, GO-Ti could enhance the neuroprotective function of astrocytes by upregulating the expression of anti-inflammatory cytokines and neuroprotective factors with improved cell adhesion and viability. Consequently, our findings suggest that GO-Ti has the potential to induce neuroprotective effects by regulating cell activity.

【 授权许可】

Unknown   
Copyright © 2023 Han, Jang, Lee, Oh, Lee and Yoon.